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Purification of Cordycepin from Medium Residues by Macroporous Resin |
LI Cong-zhen, MAO Ning |
Fujian Normal University, Industrial Microbial Fermentation Technology and Area Joint National Engineering Research Center, Fuzhou 350108, China |
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Abstract Seperating cordycepin from cordycep manual medium residues by macroporous resin, Filter out a suitable macroporous resin NKA-Ⅱ with HPLC. pH, eluent concentration of ethanol and other factors were researched to the influence of absorbance and desorption of the resin. When the pH value is 9, Adsorption velocity is 2BV/h, the resin has better absorption capacity which reach 16.5mg/g. When the eluent concentration of ethanol is 50% and the volume is 2.5 times of the resin bed, the resin has better desorption capacity which can reach 95%.
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Received: 12 November 2013
Published: 25 January 2014
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