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| The Construction of Recombinant Adenovirus Delivering Chimeric Neurturin and Activity Assay in vitro |
| WANG Wen-ju,SUN Mao-sheng,YAN Min,LI Hong-jun |
| Institute of Medical Biology, Chinese Academy of Medical Science & Peking Union Medical College, Kunming 650118, China |
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Abstract Neurturin is a member of neurotrophic family, and the gene engineering product of the Neurturin can promote the survival of the dopaminergic neurons, which is a new approach to the therapy of Parkinson’s disease. However, a few researches have indicated that the wild type of Neurturin cannot be processed and secreted into the medium. Therefore, the study intends to rebuilt the gene of wtNeurturin and replaced the signal peptide of wtNeurturin by the counterpart of NGF. The hybrid Neurturin was inserted into the genome of adenovirus and packaged in AD-293 cells. The chimeric Neurturin could be expressed in cytoplasm of vero cells and be processed and secreted, which was detected by immunofluorescence and Western blotting. Finally, the culture of dorsal root ganglia of chick embryo test had indicated that Neurturin expressed by chimeric gene had good biological activity.
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Received: 05 May 2010
Published: 25 August 2010
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