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中国生物工程杂志

China Biotechnology
China Biotechnology  2009, Vol. 29 Issue (03): 20-24    DOI:
    
The Immune Response Against Avian Infectious Bronchitis Virus Induced by Multi-epitope based Peptide Vaccines
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Abstract  

In this study, peptide vaccine was designed based on B cell and T cell epitope of IBV, and the multi-epitope based peptide vaccine was expressed in E.coli fused with glutathione S-transferase (GST). ELISA and Western blot analysis showed that purified fusion proteins (EpiA) had an excellent immune activity with chicken anti-IBV serum. The expressed fusion proteins were purified and emulsified with Freund adjuvant. Vaccination twice at an interval of 2 weeks with the emulsified the fusion proteins elicited anti-IBV specific antibodies and specific cellular responses and 73% of the vaccinated chickens were protected against mortality. We proposed use of a biosynthesis system instead of peptide synthesis, provided a new way to prepare multi-epitope based peptide vaccine



Key wordsIBV;Peptide vaccine;Design;immunogenic     
Received: 05 November 2008      Published: 31 March 2009
Cite this article:

YANG Tai- Wang-Gong-Ning- Hong-Xue- Gao-Rong- Tang-Dun-Ni- Li-Yu-Ling- Guo-Zi-Cheng. The Immune Response Against Avian Infectious Bronchitis Virus Induced by Multi-epitope based Peptide Vaccines. China Biotechnology, 2009, 29(03): 20-24.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2009/V29/I03/20

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