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| Advancement in genetic engineering for production of succinic acid by escherichia coli |
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Abstract Abstract Bio-based succinic acid could release the dependence of petroleum that is non-renewable resource, and is benefit for society, economy and environment. Escherichia coli naturally produces succinic acid as a minor fermentation product, however, its genetic background is quite clear and modified easily by genetic engineering for bio-based succinic acid industrialization. In recent years, researchers focused on the metabolic pathway of succinic acid, and a variety of genetic approaches were developed to reconstruct Escherichia coli, such as overexpression of the endogenous or heterologous CO2-fixing enzymes in Escherichia coli directed the carbon flow to succinic acid, inactivation of the competing pathways of succinic acid production, as well as construction of the glyoxylate shunt and aerobic system for succinic acid production, the production of succinic acid was increased significantly. Here, the advancement of succinic acid production in Escherichia coli by genetic engineering was reviewed.
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Received: 24 December 2008
Published: 28 July 2009
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Corresponding Authors:
ZHANG Yu-Xiu
E-mail: zhangyuxiu@cumtb.edu.cn
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