|
|
|
| The progress of inclusion body isolation and chromatographic refolding,purification methods |
|
|
|
Abstract Abstract Expression of recombinant proteins in Escherichia coli often results inclusion bodies, an aggregation form of inactive proteins. Functional proteins can only be gained through a series of steps including washing, solubilization and refolding. The inclusion body purification strategy and chromatographic refolding techniques including size exclusion chromatography, affinity chromatography, ion-exchange chromatography, hydrophobic interaction chromatography, immobilized liposome chromatography, and expanded bed adsorption chromatography were summarized in this review.
|
|
Received: 20 October 2008
Published: 28 July 2009
|
|
|
|
|
| ［1］ Lilie H, Schwarz E, Rudolph R. Advances in refolding of proteins produced in E.coli. Current Opinion in Biotechnology, 1998, 9(5): 497~501
［2］ Singh S, Panda A. Solubilization and refolding of bacterial inclusion body proteins. Journal of bioscience and bioengineering, 2005, 99(4): 303~310
［3］ 龚平生, 罗贵民. 包涵体复性研究进展(英文). 中国生物工程杂志, 2003, 23(12): 73~77
Gong P, Luo G.China Biotechnology, 2003, 23(12): 73~77
［4］ 方敏, 黄华樑. 包涵体蛋白体外复性的研究进展. 生物工程学报, 2001, 17(6): 608~612
Fang M, Huang H L. Chinese Journal of Biotechnology, 2001, 17(6): 608～612
［5］ Rinas U, Bailey J. Protein compositional analysis of inclusion bodies produced in recombinant Escherichia coli. Applied microbiology and biotechnology, 1992, 37(5): 609~614
［6］ Carrio M, Villaverde A. Construction and deconstruction of bacterial inclusion bodies. Journal of Biotechnology, 2002, 96(1): 3~12
［7］ Kane J, Hartley D. Formation of recombinant protein inclusion bodies in Escherichia coli. Trends in biotechnology, 1988, 6: 95~101
［8］ Jungbauer A, Kaar W. Current status of technical protein refolding. Journal of Biotechnology, 2007, 128(3): 587~596
［9］ Clark E. Protein refolding for industrial processes. Current Opinion in Biotechnology, 2001, 12(2): 202~207
［10］ Haase-Pettingell C, King J. Formation of aggregates from a thermolabile in vivo folding intermediate in P22 tailspike maturation. A model for inclusion body formation. Journal of Biological Chemistry, 1988, 263(10): 4977~4983
［11］ Fink A. Protein aggregation: folding aggregates, inclusion bodies and amyloid. Folding and design, 1998, 3(1): 9~23
［12］ Taylor G, Hoare M, Gray D, et al. Size and density of protein inclusion bodies. Nature Biotechnology, 1986, 4(6): 553~557
［13］ Gu Z, Weidenhaupt M, Ivanova N, et al. Chromatographic methods for the isolation of, and refolding of proteins from, Escherichia coli inclusion bodies. Protein expression and purification, 2002, 25(1): 174~179
［14］ Batas B, Schiraldi C, Chaudhuri J. Inclusion body purification and protein refolding using microfiltration and size exclusion chromatography. Journal of Biotechnology, 1999, 68(2-3): 149~158
［15］ Lin G, Lian Y, Ryu J, et al. Expression and purification of His-tagged flavonol synthase of Camellia sinensis from Escherichia coli. Protein expression and purification, 2007, 55(2): 287~292
［16］ Al-Samarrai T, Kirk C, Jones W, et al. Expression in Escherichia coli and in vitro refolding of the plant transcription factor Arabidopsis thaliana RGL3. Protein expression and purification, 2007, 53(2): 289~292
［17］ White C, Chen Q, Kenyon G, et al. A novel activity of OmpT. Journal of Biological Chemistry, 1995, 270(22): 12990~12994
［18］ Gavit P, Better M. Production of antifungal recombinant peptides in Escherichia coli. Journal of Biotechnology, 2000, 79(2): 127~136
［19］ Schlegl R, Iberer G, Machold C, et al. Continuous matrix-assisted refolding of proteins. Journal of Chromatography A, 2003, 1009: 119~132
［20］ Machold C, Schlegl R, Buchinger W, et al. Continuous matrix assisted refolding of α-lactalbumin by ion exchange chromatography with recycling of aggregates combined with ultradiafiltration. Journal of Chromatography A, 2005, 1080(1): 29~42
［21］ Reh G, Spelzini D, Tubío G, et al. Partition features and renaturation enhancement of chymosin in aqueous two-phase systems. Journal of Chromatography B, 2007, 860(1): 98~105
［22］ Cho T, Ahn S, Lee E. Refolding of protein inclusion bodies directly from E.coli homogenate using expanded bed adsorption chromatography. Bioseparation, 2001, 10(4): 189~196
［23］ Sharma K, Babu P, Sasidhar P, et al. Recombinant human epidermal growth factor inclusion body solubilization and refolding at large scale using expanded-bed adsorption chromatography from Escherichia coli. Protein expression and purification, 2008, 60(1): 7~14
［24］ Felinto M, Parra D, Lugao A, et al. Magnetic polymeric microspheres for protein adsorption. Nuclear Inst and Methods in Physics Research, B, 2005, 236: 495~500
［25］ Lin Y, Shih J, Hsu C, et al. Renaturation and Stabilization of the Telomere-Binding Activity of Saccharomyces Cdc13 (451–693) Pby l-Arginine. Analytical Biochemistry, 2001, 294(1): 44~47
［26］ Wu Q, Li F, Zhu W, et al. Cloning, expression, purification, and characterization of arginine kinase from Locusta migratoria manilensis. Comparative Biochemistry and Physiology, Part B, 2007, 148(4): 355~362
［27］ Mukhopadhyay A. Reversible protection of disulfide bonds followed by oxidative folding render recombinant hCGβ highly immunogenic. Vaccine, 2000, 18(17): 1802~1810
［28］ Swietnicki W. Folding aggregated proteins into functionally active forms. Current Opinion in Biotechnology, 2006, 17(4): 367~372
［29］ Geng X, CHANG X. High-performance hydrophobic interaction chromatography as a tool for protein refolding. Journal of chromatography, 1992, 599: 185~194
［30］ Gu Z, Su Z, Janson J. Urea gradient size-exclusion chromatography enhanced the yield of lysozyme refolding. Journal of Chromatography A, 2001, 918(2): 311~318
［31］ Liu H, Chang C. Chaperon solvent plug to enhance protein refolding in size exclusion chromatography. Enzyme and Microbial Technology, 2003, 33(4): 424~429
［32］ Park B, Lee C, Koo Y. Development of novel protein refolding using simulated moving bed chromatography. Korean Journal of Chemical Engineering, 2005, 22(3): 425~432
［33］ Ito M, Nagata K, Kato Y, et al. Expression, oxidative refolding, and characterization of six-histidine-tagged recombinant human LECT2, a 16-kDa chemotactic protein with three disulfide bonds. Protein expression and purification, 2003, 27(2): 272~278
［34］ Yin S, Zheng Y, Tien P. On-column purification and refolding of recombinant bovine prion protein: using its octarepeat sequences as a natural affinity tag. Protein expression and purification, 2003, 32(1): 104~109
［35］ Lemercier G, Bakalara N, Santarelli X. On-column refolding of an insoluble histidine tag recombinant exopolyphosphatase from Trypanosoma brucei overexpressed in Escherichia coli. Journal of Chromatography B, 2003, 786(1-2): 305~309
［36］ 刘红妮, 王彦, 龚波林,等. 高效弱阳离子交换色谱法对脲还原变性溶菌酶的折叠研究. 化学学报, 2005, 63(007): 597~602
Liu H, Wang Y, Gong B, et al. Acta Chimica Sinica, 2005, 63(7): 597～602
［37］ Langenhof M, Leong S, Pattenden L, et al. Controlled oxidative protein refolding using an ion-exchange column. Journal of Chromatography A, 2005, 1069(2): 195~201
［38］ Machold C, Schlegl R, Buchinger W, et al. Matrix assisted refolding of proteins by ion exchange chromatography. Journal of Biotechnology, 2005, 117(1): 83~97
［39］ Lu H, Zang Y, Ze Y, et al. Expression, refolding, and characterization of a novel recombinant dual human stem cell factor. Protein expression and purification, 2005, 43(2): 126~132
［40］ 耿信笃, 白泉. 用疏水色谱复性并同时纯化蛋白质的机理及其应用. 中国科学(B辑), 2002, 32(5): 460~471
Geng X D, Bai Q. Science in China (Series B), 2002, 32(5): 460～471
［41］ Yoshimoto M, Shimanouchi T, Umakoshi H, et al. Immobilized liposome chromatography for refolding and purification of protein. Journal of Chromatography B: Biomedical Sciences and Applications, 2000, 743: 93~99
［42］ Cabanne C, Noubhani A, Hocquellet A, et al. Purification and on-column refolding of EGF Poverexpressed as inclusion bodies in Escherichia coli with expanded bed anion exchange chromatography. Journal of Chromatography B, 2005, 818(1): 23~27
［43］ Harrowing S, Chaudhuri J. Effect of column dimensions and flow rates on size-exclusion refolding of β-lactamase. Journal of biochemical and biophysical methods, 2003, 56: 177~188
［44］ Meng F, Park Y, Zhou H. Role of proline, glycerol, and heparin as protein folding aids during refolding of rabbit muscle creatine kinase. International Journal of Biochemistry and Cell Biology, 2001, 33(7): 701~709
［45］ Chen Y, Huang L, Chiu H, et al. Temperature-responsive polymer-assisted protein refolding. Enzyme and Microbial Technology, 2003, 32(1): 120~130
［46］ Dong X, Yang H, Sun Y. Lysozyme refolding with immobilized GroEL column chromatography. Journal of Chromatography A, 2000, 878(2): 197~204
［47］ 王君, 林莹, 卢滇楠等. CTAB 辅助溶菌酶复性过程动力学. 化工学报, 2004, 55(9): 1481~1487
Wang J, Lin Y, Lu D, et al. Journal of Chemical Industry and Engineering (China), 2004, 55(9): 1482～1487
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
