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中国生物工程杂志

China Biotechnology
China Biotechnology  2009, Vol. 29 Issue (07): 43-49    DOI:
    
cDNA cloning and bioinformatic analysis of SAMS gene from porphyra yezoensis
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Abstract  Abstract Drought, salinity and low temperature are the most abiotic stresses that make agriculture production reduction. The S-Adenosylmethionine synthetase (SAMS) gene (SAMS) is closely connected to tolerance to abiotic stresses. Furthermore, SAMS-overexpressing plant can be more tolerant to drought, salinity and low temperature. So investigation of SAMS is important to illustrate mechanism of tolerance to abiotic stresses, and to improve transgenic plant resistance through biotechnology. Isolation and characterization of cDNA of Porphyra yezoensis S-Adenosylmethionine synthetase gene (PySAMS) was reported. SAMS from other organisms was used as a probe to search the P. yezoensis dbEST. ESTs showing great similarity to CSase were assembled into contig. According to the open reading frame of contig, the primers were designed. Total RNA was isolated from thallus of P. yezoensis. And the cDNA sequence (GenBank accession: FJ404748) of PySAMS was isolated successfully through experiment of RT-PCR. The cDNA contains an 1155nt of continuous complete open reading frame (ORF) encoding a polypeptide of 384 amino acids with a calculated molecular mass of 41.8kDa. Sequence comparison of the PySAMS reveals high amino acid sequence identity with SAMS from other organism, such as Dictyostelium discoideum, Chlamydomonas reinhardtii, Arabidopsis thaliana and Oryza sativa.PySAMS contains conserved amino acids residue and sequences motifs closely related to function of SAMS enzyme, and possess highly similar tertiary structure with human SAMS. So like other SAMS, PySAMS plays an important role in tolerance to abiotic stresses in P. yezoensis, and is a key enzyme of cell activity.

Key wordsPorphyra yezoensis      S-Adenosylmethionine synthetase      CloningBioinformatics     
Received: 24 November 2008      Published: 28 July 2009
ZTFLH:  Q78  
Corresponding Authors: Chu-Wu Liu     E-mail: liucw@gdou.edu.cn
Cite this article:

YI Le-Fei- Wang-Ping- Zhou-Xiang-Gong- Liu-Chu-Wu. cDNA cloning and bioinformatic analysis of SAMS gene from porphyra yezoensis. China Biotechnology, 2009, 29(07): 43-49.

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https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2009/V29/I07/43

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