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| Effect of Surfactants on Extracellular Production of α-Cyclodextrin Glycosyltransferase from E.coli |
| DING Run-rong1,2, LI Zhao-feng1,2, LI Jin-gen1, LU Chen-xia1, WU Jing1,2, CHEN Jian1,2 |
| 1 State Key Laboratory of Food Science and Technology,Jiangnan University, Wuxi214122, China
2 School of Biotechnology,The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi214122, China
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Abstract Abstract The effect of surfactants on the production of recombinant extracellular α-CGTase in E.coli was studied. The result indicated that the optimum concentration of Tween-80 and Triton X-100 are 2% and 0.5% when addition time of surfactant was performed at the beginning of culture. In such condition, α-CGTase activity of culture supernatant reached 2.03 U/ml for Tween-80 and 4.92 U/ml for Triton X-100 and were respectively 4.6 and 12.67 higher than that without addition of surfactant. The different addition time during culture stage could not further improve the yield. When adding 0.02％ SDS at 20 h culture, the final yield of enzyme could reach to 5.31 U/ml, which was 12.75 times higher than that of control. The enhanced extracellular secretion of α-CGTase by surfactants is possibly caused by the increasing of the cell membrane pemeabilization of E.coli.
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Received: 24 February 2009
Published: 28 July 2009
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