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| Regulation of a highly repeated GT sequence from DCA1 promoter of dunaliella salina in salt-induction |
| LUO Qing-juLI JieYAN Hong-xiaLU Xue-jingLU Yu-minXUE Le-xun |
| (Biology Department,Zhengzhou University,Zhengzhou450001,China) |
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Abstract Abstract To identify whether a highly repeated GT sequence from DCA1 promoter from Dunaliella salina, which have been proved to be a salt-inducible promoter in our previous study, would be a salt-inducible regulation element, different primers were designed to amplify 6 different-length fragments of DCA1 promoter from D.salina by PCR. After these fragments were respectively inserted into the HindⅢ-BamH I sites of the vector pUΩGUS, serial expression vectors containing the gus gene were generated.D.salina cells transformed with these recombinant plasmids by electroporation were grown in liquid media containing different concentrations of sodium chloride respectively. GUS enzyme activity was measured histochemically and fluorometrically. The results revealed that 3 fragments containing GT repeated sequence drove the external gus gene expression and the expression pattern of the gus gene was regulated by the concentrations of sodium chloride. Additionally, the 2 fragments without tandem GT sequence drove the gus gene expression, but the expression pattern of the gus gene wasn’t regulated by the concentration of sodium chloride; Also, the upstream fragment of the tandem GT sequence wasn’t able to drive the gus gene expression. In conclusion, the highly repeated GT sequence from the DCA1 promoter plays an important role in the salt-inducible regulation of DCA1 promoter from D.salina and might be a novel salt-inducible element.
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Received: 16 February 2009
Published: 28 July 2009
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| ［1］ Fisher M, Gokhman I, Pick U, et al. A structurally novel transferring-like protein accumulates in the plasma membrane of the unicellular green alga D.salina grown in high salinities. J Biol Chem, 1997, 272: 1565~1570
［2］ Fisher M, Pick U, Zamir A, et al. A salt-induced 60-kilodalton plasma membrane protein plays a potential role in the extreme halotolerance of the alga D.salina. Plant physiol, 1994,106(4):1359~1365
［3］ Fisher M, Gokhman I, Pick U, et al. A salt-resistant plasma membrane carbonic anhydrase is induced by salt in D.salina. J Biol Chem,1996, 271: 17718~17723
［4］ 吕玉民, 姜国忠,牛向丽, 等. 杜氏盐藻两种碳酸酐酶基因启动子的克隆和功能研究. 遗传学报, 2004, 31(10): 1157~1166
Lu Y M, Jiang G Z, Niu X L, et al.Salina. Acta Genetica Sinica,2004, 31 (10):1157~1166
［5］ 耿德贵, 王义琴, 李文彬, 等.Gus基因在杜氏盐藻细胞中的瞬间表达. 高技术通讯, 2002, 12(2): 35~39
Geng D G, Wang Y Q, Li W B, et al. Transient expression of Gus gene in D.salina. High Technology Letters,2002, 12(2): 35~39
［6］ Jefferson R A, kavanagh T A, Bevan M W.GUS fusions: B-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J, 1987, 6(13): 3901~3907
［7］ 李杰, 曲东京, 刘玲, 等. 不同启动子驱动下转基因盐藻外源基因的稳定表达. 中国生物工程杂志,2007, 27 (3): 47~53
Li J, Qu D J, Liu L, et al.China Biotechnology, 2007, 27 (3): 47～53
［8］ Thompson M A, Eunpyo M, Ung-Jin K, et al. Human indolethylamine N-methyltransferase: cDNA cloning and expression, gene cloning, and chromosomal localization. Genomics, 1999, 61: 285~297
［9］ Jiang M, Lorne A B, Andrew A P. Cloning, sequencing and expression of the CAM Pfactor gene of streptococcus uberis. Microbial Phathogenesis, 1996, 20: 297~307
［10］ 王胜华, 徐莺, 陈放, 等. 水稻花粉特异性启动子的克隆与表达. 四川大学学报(自然科学版), 2001, 38 (4): 554~559
Wang S H, Xu Y, Chen F, et al.J Sichuan University ( Nature Science edition),2001,38 (4): 554～559
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