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| Recombinant baculoviruses as mammalian cell gene-delivery vectors: a review |
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Abstract The baculovirus expression system has been used extensively for the expression of recombinant proteins in insect cells. Recently, recombinant baculoviruses containing mammalian cell-active promoter element have been used to transduce a broad spectrum of primary and established mammalian cells, including non-hepatic cells. Recombinant baculoviruses have been used successfully for transient or stable gene delivery in mammalian cells in vitro, while the efficiency of delivering gene in vivo is inhibited obviously by complements, but efforts have been made to overcome the problems, for instance, VSV-G-pseudotyped baculoviruses display complement resistance. The mechanism of the transduction is still not clearly understood, though many researchers have been done. The baculovirus is able to replicate only in insect cells, but it is incapable of initiating replication cycle in mammalian cells, which guarantees high biosafety of this gene delivery system. In addition, this system is easily manipulated and able to carry large inserts. These attributes will undoubtedly lead to the increased application and continued development of this system for highly effective gene delivery into mammalian cells.
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Received: 04 September 2006
Published: 10 May 2010
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