Improving Biocontrol Effect of Bacillus circus on Botrytis cinerea through Genetic Modification with β-1,3-glucanase

China Biotechnology

2007, Vol. 27

Issue (4): 44-49 DOI:

Improving Biocontrol Effect of Bacillus circus on Botrytis cinerea through Genetic Modification with β-1,3-glucanase

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Abstract

Bacillus cereus B-04 was isolated in zibo soil and proved as a biocontrol agent of Botrytis cinerea. The aim of this study was to enhance the biocontrol effect of this strain by introduction of a β-1,3-glucanase gene. A 4.1kb DNA fragment containing β-1,3-glucanase gene was inserted into vector pBE2 and pHY300PLK to construct a new plasmid, PBE2-glu and pHY300PLK-glu,which was introduced into Bacillus cereus B-04, resulting in a new strain named B-04-glu.Restriction digestion and β-1,3-glucanase plate culture and PCR experiment confirmed that B-04-glu contained a functional β-1,3-glucanase gene. Compared with wild type B-04 in pot experiment, B-04-glu had increased effect against Botrytis cinerea caused by tomato.

Key words： Bacillus cereus β-1 3-glucanase gene Botrytis cinerea

Received: 06 November 2006 Published: 25 April 2007

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Cite this article:

. Improving Biocontrol Effect of Bacillus circus on Botrytis cinerea through Genetic Modification with β-1,3-glucanase. China Biotechnology, 2007, 27(4): 44-49.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2007/V27/I4/44

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