Please wait a minute...

中国生物工程杂志

China Biotechnology
China Biotechnology  2007, Vol. 27 Issue (4): 94-98    DOI:
    
Expression of a kind of curcin induced from Jatropha curcas (curicin 2) in tobacco
Download: HTML   PDF(464KB) HTML
Export: BibTeX | EndNote (RIS)      

Abstract  

A new kind of curcin (curcin 2), induced by several kinds of stresses from Jatropha curcas leaves, under the control of the 35S CaMV (cauliflower mosaic virus) promoter, was introduced into tobacco genome by Agrobacterium tumefaciens-mediated transformation method. Curcin 2 protein was only detected in the transgenic tobacco plants transformed with the cur2p fragment (coding premature curcin 2 protein), but not in the plants transformed with cur2m fragment (coding mature curcin 2 protein). The transgenic lines expressing curcin 2 showed increased tolerance to tobacco mosaic virus (TMV).



Key wordsJatropha curcas      curcin      transgenic tobacco      TMV     
Received: 20 December 2006      Published: 25 April 2007
Cite this article:

. Expression of a kind of curcin induced from Jatropha curcas (curicin 2) in tobacco. China Biotechnology, 2007, 27(4): 94-98.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2007/V27/I4/94

[1] Min YAO,Shu-hua ZHU,Fo-sheng LI,Shi-yan ZHANG,Lin TANG. Analysis of Salt Tolerance and Insect Resistance of Transgenic Tobacco Expressing AtCYSa from Arabidopsis[J]. China Biotechnology, 2018, 38(4): 8-16.
[2] SU Zhi-zhe, WANG Xue-hua, YANG Hua, SUN Huan, WEI Wei. Transcriptome Analysis of Cadmium Exposed Jatropha curcas[J]. China Biotechnology, 2016, 36(4): 69-77.
[3] HAN Shuang, YANG Zhi-li, CHEN Li-mei. Over Expression of Arabidopsis CAT Improved the Absorption and Tolerance of Gas HCHO in Tobacco[J]. China Biotechnology, 2015, 35(5): 41-48.
[4] SHAO Zi-jing, JIANG Nan, YAN Hua-li, ZHAN Cheng, XU Ying, CHEN Fang. The Soluble Prokaryotic Expressed Ribosome-inactivating Protein Curcin2 with Anti-fungal Activity from Jatropha curcas L.[J]. China Biotechnology, 2013, 33(7): 43-49.
[5] LIU Lei, SUN Zhen, SONG Zhong-bang, XIAO Su-qin, CHEN Li-mei. Simultaneous Over-expressions of AOD1 and HPS-PSI in Chloroplasts Creates a Novel Photosynthetic CH3OH-assimilation Pathway and Enhances Its Ability to Assimilate CH3OH[J]. China Biotechnology, 2013, 33(12): 69-78.
[6] SONG Feng, SUN Min, LUO Ke-Meng. A Simple and Rapid PCR-based Method for Identifying Transgenic Tobacco Plants Carrying a Single Copy of the Integrated Gene[J]. China Biotechnology, 2010, 30(04): 83-88.
[7] HU Li-E, SONG Wei, LIU Xiao-Hui, JIANG Ting, DIAO Ling-Xia. Expression of Bioactive Thymosin α1 Using Marker Free Transgenic Tobacco[J]. China Biotechnology, 2010, 30(03): 1-8.
[8] . Transgenic tobacco expressing the crocus CsZCD gene showed the production of crocetin[J]. China Biotechnology, 2007, 27(6): 51-55.
[9] Pei-Qiang YAN Hong KANG Xian-Quan BAI Zhao-Kui GUO Chen-Cai CHU. Study on tobacco virus resistance with RNAi method[J]. China Biotechnology, 2007, 27(11): 27-31.