中国生物工程杂志

The effect of exogenous cell factors as mLIF, hSCF, bFGF and hIL-11 on the stage of the 19th and 28th EPGCs proliferation and differentiation were elucidated by means of MTT test when combined added in the culture medium. The regulated mechanism of those cell factors was discussed as well. When the cell factors as mLIF, hSCF, bFGF and hIL-11 combined in the medium, it showed evident effect on proliferation of stage 19th EPGCs compared to the control group when cultured for 72h (p<0.05). The proliferation of EPGCs was obviously inhibited when the concentration of mLIF and hSCF beyond 20ng/ml, the suitable concentration of mLIF was 10~20ng/ml, hSCF was 15~20ng/ml. The combined of hSCF and bFGF was more efficient than single cell factor added. The suitable concentration of bFGF was 10~20ng/ml. Similar to hIL-11, when combined with the other cell factors, the number of viable cells increased distinctly（P<0.05）, the suitable concentration of hIL-11 was 0.10~0.20ng/ml. The results would promote and facilitate development of chicken EPGCs line and also highlight a general role in stem cell proliferation mechanism.