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中国生物工程杂志

China Biotechnology
China Biotechnology  2010, Vol. 30 Issue (08): 6-12    DOI: Q819
    
The Process of Coculturing Peripheral Blood Mononuclear Cells and Human Umbilical Cord Derived Mesenchymal Stem Cells Observed with Atomic Force Microscope
GONG Chao-qun1,CAI Ji-ye1,GAO Shi-jun2,WANG Qiu-lan1,JIN Hua1
1.Department of Chemistry, College of Life Science and Technology , Jinan University, Guangzhou 510632,China
2.Department of Microbiology and Immunology, Medical College, Jinan University, Guangzhou 510632, China
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Abstract  

The inverted microscope was used to observe the process that peripheral blood mononuclearcells (PBMCs)  were cocultured with allogeneic human umbilical cord derived mesenchymal stem cells ( hUC- MSCs). The atomic force microscope was used to analyze the morphology and mechanical property after two kinds of cells were cocultured. The result showed that the morphology of adherence PBMCs changed greatly after cocultured, and was coated with different grain sizes of polymers. From the high spatial resolution of AFM force-curves, the adhesion force and stiffness values of PBMCs cocultured with hUC-MSCs were stronger than those of controlled PBMCs. CCK-8 indicated that PBMCs may appear to compete with hUC- MSCs in the co-culture system. The visualized data obtained from AFM can provide a better understanding the interactive mechanism of hUC-MSCs and PBMCs.



Key wordsHuman umbilical cord mesenchymal stem cells      Peripheral blood mononuclear cells      Atomic force microscope (AFM)      Inverted microscopemicroscope     
Received: 16 November 2009      Published: 25 August 2010
Cite this article:

GONG Chao-Qun, CA Ji-Ye, GAO Shi-Juan, WANG Qiu-Lan, JIN Hua. The Process of Coculturing Peripheral Blood Mononuclear Cells and Human Umbilical Cord Derived Mesenchymal Stem Cells Observed with Atomic Force Microscope. China Biotechnology, 2010, 30(08): 6-12.

URL:

https://manu60.magtech.com.cn/biotech/Q819     OR     https://manu60.magtech.com.cn/biotech/Y2010/V30/I08/6

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