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EST technology and its application in mammalian embryo research |
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Abstract EST (expressed sequence tag,EST) is approximately 150~500 bp sequence of the gene expression exogenous sequence fragment,and randomly selected from large-scale cDNA sequencing of the genome of the cells or expressed sequence tags. EST represents a certain period of a biological cell or a gene expression. This paper reviews the technical principles EST, early mammalian embryo research and the theoretical foundation of early embryo research EST in the application, EST to discuss the study and analysis of the development trends.
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Received: 01 March 2007
Published: 25 August 2007
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