Cultural Conditions for Production of Glutathione by Mutant Saccharomyces J-X25

China Biotechnology

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Cultural Conditions for Production of Glutathione by Mutant Saccharomyces J-X25

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Abstract The production conditions of Glutathione with shaking flask fermentation by the mutant Saccharomyces J-X25，a methionine-defected strain were studied, and the optimum culture conditions are as follow: initial pH6.0，temperature 30℃，100ml in 500ml flask，the inoculum size 10% and agitation rate 220r/min. Our study emphasizes on the stimulating effect on the cells by dioxogen and the sodium lactate as surfactant both of which were added in the logarithmic phase of fermentation, total producing GSH 0.253g/L , 52% higher than the control experiment without the additions. Compared with the initial strain，the production of GSH by the mutant in optimum conditions was increased by 79%.

Key words： Culture conditions Dioxogen Sodium lactate Glutathione

Received: 21 November 2005 Published: 25 July 2006

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. Cultural Conditions for Production of Glutathione by Mutant Saccharomyces J-X25. China Biotechnology, 2006, 26(07): 48-51.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2006/V26/I07/48

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