HPLC／MS Analysis of the Content of Collagen Type I and III in Rat Skin

China Biotechnology

2007, Vol. 27

Issue (4): 71-76 DOI:

HPLC／MS Analysis of the Content of Collagen Type I and III in Rat Skin

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Abstract

Abstract: The main collagens in skin are type I and III. In this research, total collagens were separated from rat skin by enzymatic digest and acetum methods. The collagens were denatured at 60℃ and digested with trypsin. Characteristic peptides typical for collagen type I and III were identified with high performance liquid chromatography/mass spectrometry. The relative abundance of collagen type I/III was determined according to characteristic peptides. The result indicated that the content of collagen type III in rat skin decreased with growth stage, and content of collagen type I increased slightly with growth stage, the ratio of collagen type I/III keep stable after 8 weeks.

Key words： Collagen separation enzymatic degradation High performance liquid chromatography mass spectrometry

Received: 14 December 2006 Published: 25 April 2007

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. HPLC／MS Analysis of the Content of Collagen Type I and III in Rat Skin. China Biotechnology, 2007, 27(4): 71-76.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2007/V27/I4/71

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