中国生物工程杂志

Differential display is the available method used for comprehending the genic expression of different cells or the same cells in different periods or stages. Because of its simplicity, sensitivity, and reproducibility，it has become the most widely used technique for studying differential gene expression This paper summarized the principle of differential display technique and emphasized the relative techniques, which include the extraction and qualification of mRNA, RT, electrophoresis, reamplification，confirmation, sequencing and so on.At last, it introduced the status of this technique used in cancer research,detection of pathology related genes，display of cells growth related genes and the expression of cells gene induced by diferrent factors.