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| Studies on the isolation, purification and biological activity of the polysaccharides PNM from Phellinus nigiricans |
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Abstract The crude polysaccharide PNM was extracted from the submerged culture of Phellinus nigiricans by the method of ethanol precipitation. PNW is a heteropolysaccharide and GC analysis indicated that PNM consisted of xylise, mannose, galactose and glucose in the quality radio 0.05：0.63：0.31：1.00. PNM was purified by freeze thawing, methods of Sevag and preparation on Sepharose CL-6B column chromatography, it was PNMⅠ. HPLC analysis manifested that it was uniform fraction and the moleculer weight was about 29 throusand. Initially investigated the effects on the lymphocyte proliferation in vitro. The results were that both PNM and PNMⅠhad the most significant effects on the proliferation of mouse spleen cell in vitro at 400μg/ml.
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Received: 10 November 2006
Published: 25 April 2007
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