Detection of Cellular Binding Protein of Hepatitis B virus PreS1 Domain on HepG2 Cell Membane

China Biotechnology

2009, Vol. 29

Issue (09): 0-0 DOI:

Detection of Cellular Binding Protein of Hepatitis B virus PreS1 Domain on HepG2 Cell Membane

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Abstract To search for novel HBV preS1-interacting proteins on HepG2 cell membrane, preS1 protein was recombined with GST tag and GST pull down assay was employed to detection of preS1 domain cellular binding protein, using the precleared lysate of biotinylated HepG2 cells as a source of receptor molecules. Our results showed that an approximately 110-kDa protein (p110) was detected to bind to preS1. Tissue distribution analysis further indicated that p110 binding is restricted to human hepatocytes. The data demonstrated that p110 is a binding protein to preS1 on HepG2 cell membrane.

Key words： Hepatitis B virus preS1 domain binding protein pull down

Received: 08 April 2009 Published: 10 May 2010

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	DING Gang-Jiang
	ZHANG Jun
	YANG Feng
	YANG Jian
	XIANG Guang-Meng
	TANG Ni
	HUANG Ai-Long

Cite this article:

. Detection of Cellular Binding Protein of Hepatitis B virus PreS1 Domain on HepG2 Cell Membane. China Biotechnology, 2009, 29(09): 0-0.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2009/V29/I09/0

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