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| Nucleic Acid Sequence-Based Amplification and Its Applications in Viral Diagnosis |
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Abstract Abstract：Nucleic acid sequence-based amplification (NASBA) is a sensitive, isothermal, transcription-based amplification system specifically designed for the detection of RNA targets, which could amplify templete RNA in 2h under isothermal condition at about 42°C and without any special equipment. NASBA is now widely applicated in diagnosis of many pathogenic microorganism. This article is mainly about principles and applications of NASBA in viral diagnosis.
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Received: 19 June 2008
Published: 25 January 2009
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