Development and application of RNAi interference libraries in functional genomics

China Biotechnology

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Development and application of RNAi interference libraries in functional genomics

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Abstract RNA interference (RNAi) is a process in which double-stranded RNA (dsRNA) induces specific postranscriptional silencing of homologous transcripts. Systematic silencing of genes on a genome-wide scale by using RNAi library targeting many thousands of genes has been providing a powerful research tool in functional genomics. RNAi libraries, which may be derived from plasmids cloning, virus packaging, PCR amplification, chemically synthesis or enzymatic digestion, have been successfully used to gene function identification, signaling pathway dissection and drug target discovery, etc. As promising progresses have been made in this field, the development, application and problems of RNAi library methodology and future prospects were reviewed in this paper.

Key words： RNAi library functional genomics high-throughput screening

Received: 21 February 2006 Published: 25 July 2006

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. Development and application of RNAi interference libraries in functional genomics. China Biotechnology, 2006, 26(07): 84-89.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2006/V26/I07/84

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