中国生物工程杂志

Klebsiella oxytoca ME-UD-3-4 with high glucose tolerance was obtained from the original strain ME-UD-3 after mutagenesis by UV coupled with diethyl sulfate and following continous subculture in the broth with gradually increased glucose concentration. Compared with the original strain which could only endure 120 g/L glucose, the mutant was able to tolerate as high as 300g/L glucose. 95 g/L glucose was depleted by the mutant in 40h,which was 8h shorter than that of the original strain; and the final 2,3-butanediol concentration and productivity of which achieved to 43.0g/L and 1.08 g/L·h, respectively, with the yield up to 91% of the theoretical yield.