Prokaryotic expression and acetylation assays of histone acetyltransferase PCAF

China Biotechnology

2009, Vol. 29

Issue (01): 12-16 DOI:

Prokaryotic expression and acetylation assays of histone acetyltransferase PCAF

Wei-Zhong Li

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Abstract

P300/CBP-associated factor (PCAF), an important member of histone acetyltransferase family (HATs) within eukaryotic cells, is capable of inducing the acetylation of histone, promoting the transcription of specific genes and involving in many biological effects. In the present study, fulllength cDNA of PCAF was inserted into plasmid pGEX-5x-1, then the soluble protein GST-PCAF was expressed in E.coli BL21(DE3) after the optimization of inducing conditions. The recombinant protein was further purified with affinity chromatography and tested the activity by in vitro acetylation assays. High efficient PCAF protein produced by this method could serve for the study on the role of PCAF in gene regulation and the interaction between PCAF and other proteins

Key words： p300/CBP-associated factor Prokaryotic expression Acetylation assays

Received: 12 August 2008 Published: 25 January 2009

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Cite this article:

Wei-Zhong Li. Prokaryotic expression and acetylation assays of histone acetyltransferase PCAF. China Biotechnology, 2009, 29(01): 12-16.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2009/V29/I01/12

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