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Primary Study of Producing UMP by Overexpressing URA5 and URA3 Genes in Saccharomyces cerevisiae |
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Abstract To increase the biotransfomation efficiency from the orotic acid to the Uridine 5’-monophosphate(UMP), URA5 gene encoding Orotate phosphoribosytransferase was amplified from Saccharomyces cerevisiae BY4742 by PCR, then it was inserted into the expression vector pYX212(contained orotidine monophosphate decarboxylase gene URA3)and the pYX212-URA5 was transformed into Saccharomyces cerevisiae BJX12 by electroporation. The recombinant strain was elementarily used to convert orotic acid to UMP. The results showed that pYX212-URA5/BJX12 could accumulate 7mM UMP from 32mM orotic acid in 26h, significantly higher than both control groups pYX212/BJX12 ( 2.7mM ) and BJX12 ( 2.4 mM )
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Received: 25 July 2008
Published: 20 April 2009
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