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Establishment of Two-dimensional Electrophoresis System for Proteome of Monascus ruber |
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Abstract Two-dimensional electrophoresis (2-DE) is an important technique in proteomics research. The 2-DE system for proteome of Monascus ruber was established by comparing and analyzing the infection caused by different kinds of mediums, lysis buffer and the condition of rehydration. By cultivating the Monascus ruber with YES for 6 days, extracting total protein by TCA-acetone, lysis buffer with 8 mol/L urea, 2 mol/L thiourea, 4 % CHAPS, 1 % DTT and 2 % Bio-lyte, an ideal 2-DE map with higher resolution and better legibility was obtained, which laid a foundation for the further studies on proteome of Monascus ruber.
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Received: 27 November 2008
Published: 27 April 2009
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[1] 纪远中.红曲及红曲霉的研究现状及进展.天津药学,2005,17(2):65~68
Ji Y Z.Tianjin Pharmacy, 2005, 17(2): 65~68
[2] 成海平,钱小红.蛋白质组研究的技术体系及其进展.生物化学与生物物理进展,2000,27(6):584~588
Cheng H P, Qian X H.Prog Biochem Biophys, 2000, 27(6): 584~588
[3] 文李,刘盖.水稻花粉总蛋白质双向凝胶电泳方法建立及应用.中国水稻科学,2007,21(1):13~19
Wen L, Liu G,.Chinese J Rice Science, 2007, 21(1): 13~19
[4] 鲁子贤.蛋白质和酶学研究方法.第1版.北京:科学出版社,1989
Lu Z X.Research Methods for Protein and Enzymology.1st ed. Beijing:Science Press, 1989
[5] 傅亮.红曲色素在红曲霉发酵代谢中生理功能的探讨.食品科学,1998,226(19):10~12
Fu L.Food Science, 1998, 226(19): 10~12
[6] 夏其昌,曾嵘.蛋白质化学与蛋白质组学.第1版.北京:科学出版社,2004
Xia Q C, Zeng R, et al.Protein Chemistry and Proteomics. 1st ed.Beijing:Science Press, 2004
[7] 许杨,李燕萍.不同培养条件对红曲霉产桔霉素影响的研究.食品工业科技,2002,23(10):33~35
Xu Y, Li Y P.Science and Technology of Food Industry, 2002, 23(10): 33~35
[8] Lin W Y, Chang J Y.Proteome response of Monascus pilosus during rice starch limitation with suppression of monascorubramine production.Journal of Agricultural and Food Chemistry,2007, 55(22): 9226~9234
[9] 许赣荣,李凤琴.红曲霉桔霉素的检测方法及红曲霉产桔霉素的判别方法.微生物学通报,2004,31(3):16~20
Xu G R, Li F Q.Microbiology, 2004, 31(3): 16~20
[10] Rabilloud T, Adessi C.Improvement of the solubilization of proteins in twodimensional electrophoresis with immobilized pH gradients.Electrophoresis, 1997, 18: 307~316
[11] Grog A, Weiss W.Current twodimensional electrophoresis technology for proteomics.Proteomics, 2004, 4: 3665~3685
[12] Kyla Pennington,Emma Mc Gregor.Optimization of the first dimension for separation by twodimensional gel electrophoresis of basic proteins from human brain tissue.Proteomics,2004,4(1):27~30 |
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