Abstract The serial analysis of gene expression (SAGE) technology is a very useful skill to do the researches on gene. It has many advantages, such as high throughout, high-fidelity, qualitation and quantitation. Based on our own researches and combined with the reviews of the related literature at home and abroad, we analyzed and evaluated the latest development of the research on SAGE, especially the development of principles, methods and applications of the technology. The improvements include three aspects, the optimization of SAGE itself, analysis among several SAGE libraries, combining with other gene researching methods. Through the whole view of SAGE used in liver researches, we find that the SAGE technology has been improved to some degree, especially, it's applications in many fields are greatly extended. The principles of SAGE change little, the methods of SAGE has been improved a lot.
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