Screening of Fungi for Microbial Oil Production Using N-Acetyl-D-glucosamine

China Biotechnology

2008, Vol. 28

Issue (11): 58-62 DOI:

Screening of Fungi for Microbial Oil Production Using N-Acetyl-D-glucosamine

Download:

HTML

PDF(471KB) HTML
Export: BibTeX | EndNote (RIS)

Abstract

Preliminary screening study on 21 fungi strains were carried out to identify those capable of producing lipids using N-acetyl-D-glucosamine (NAG) as carbon source. It was found that 7 strains can assimilate NAG effectively, and 3 of them can accumulate substantial amount of lipids. Under shaking flask culture conditions, cellular lipid content for C. albidus ATCC 56298 and T. fermentans CICC 1368 reached 67% and 48%, respectively. Gas chromatography analysis indicated that compositional fatty acids of these microbial lipid samples contained mainly palmic acid, stearic acid and oleic acid, closely resembling those of vegetable oil. This work further extends feedstock options for microbial lipid fermentation and provides new ideas for exploration of chitin and related renewable materials.

Key words： Oleaginous microorganisms Microbial lipids Chitin N-Acetyl-D-glucosamine

Received: 23 June 2008 Published: 20 April 2009

	Service
	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	WU Sai-Guo
	DIAO Xin
	HU Cui-Min
	ZHANG Su-Fang
	HUA Yan-Yan
	DIAO Zong-Bao

Cite this article:

. Screening of Fungi for Microbial Oil Production Using N-Acetyl-D-glucosamine. China Biotechnology, 2008, 28(11): 58-62.

URL:

https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2008/V28/I11/58

[1]	Min-hua XU,Jing-jing ZHANG,Xiao-bao JIN,Xia-bo LI,Yan WANG,Yan MA. Cloning\Expression and Bioactivity of the Chitinase Gene ChiA from the Endophytes of Periplaneta americana[J]. China Biotechnology, 2019, 39(1): 31-37.

[2]	Si-ming JIAO,Gong CHENG,Yu-chen ZHANG,Cui FENG,Li-shi REN,Jian-jun LI,Yu-guang DU. *Expression of Chitinase from Trichoderma reesei* and Analysis the Composition and Structure of its Hydrolysates**[J]. China Biotechnology, 2018, 38(10): 30-37.

[3]	WANG Ya nan, SHEN Hong wei, YANG Xiao bing, ZHAO Zong bao. *Effects of Lipid Production by Rhodosporidium toruloides* under Conditions with Limitation of Different Nutrient Elements**[J]. China Biotechnology, 2016, 36(11): 16-22.

[4]	ZHANG Rui-xiang, YANG Zhong . Screening, Identification and Enzymatic Properties Studies of Chitinase from Submarine Sediments[J]. China Biotechnology, 2015, 35(8): 76-82.

[5]	LIU Ya-qing, HOU Xiao-lun, GUO Wei-ting, CHANG Zhong-yi, GAO Hong-liang, BU Guo-jian, LU Wei, XIE Xiu-juan, JIN Ming-fei. *Enhancement of Transglutaminase Production in Streptoverticillium mobaraensis* as Achieved by Treatment with Chitin**[J]. China Biotechnology, 2015, 35(6): 40-45.

[6]	WANG Hao, WU Li, ZHU Xiao-hua, LIU Wang-wang, YANG Gong-ming. Progress and Prospect of Chitin Deacetylase[J]. China Biotechnology, 2015, 35(1): 96-103.

[7]	SHI Xian-wei, ZHANG Wei-tao, ZHANG Xiao-fei, YANG Guang-yu, FENG Yan. *The Heterologous Expression and Characterization of Lytic Polysaccharide Monooxygenase from Actinosynnema mirum* DSM 43827**[J]. China Biotechnology, 2014, 34(7): 17-23.

[8]	ZHANG Jun-xia, CONG Da-peng, LI Ya-hua, XIAN Hong-quan. *Prokaryotic Expression of tachi2* Gene from Trichoderma asperellum and Characterization of Recombinant Enzyme**[J]. China Biotechnology, 2013, 33(6): 45-51.

[9]	LIN Zhen-ya, WU Qiong, SUN Rui-yan, CHEN Jie, LI Ya-qian. *Optimization of Fermentation Conditions of Collaborative Expression of Chitinase and Natamycin from Streptomyces lydicus* A01-chit33 CT**[J]. China Biotechnology, 2012, 32(10): 67-73.

[10]	AI Zuo-zuo, YAN Ri-ming, YUAN Jin-yun, ZHANG Zhi-bin, ZHU Du. Optimization of Single Cell Oil Produced from Cassava Starch by Response Surface Methodology[J]. China Biotechnology, 2012, 32(07): 66-72.

[11]	XI You-wei, XIA Tian, ZHAO Ya-ling, JIA Jing-fang. Purification and Toxicological Studies of HaSNPV Chitinase[J]. China Biotechnology, 2011, 31(02): 43-49.

[12]	JIA Qi-Yu, WANG Yu-Guang, SUN Jian-Bei, LEI Xue-Hua, GU Wen-Liang. Isolation of a Strain of Endophytic Bacteria against Banana Fusarium Wilt and Activity Analysis of Signal Peptide of its Chitinase[J]. China Biotechnology, 2010, 30(09): 24-30.

[13]	ZHANG Hai-Chao, WANG Ting, TIAN Nan, LIU Xin-Li. Research on Screening , Identification and Enzyme Producing Characteristics of Chitinase Producer[J]. China Biotechnology, 2010, 30(08): 82-87.

[14]	HAN Wen-Xia, CHEN Li, CHEN Liang, GONG Ji, CHEN Wu-Ling. The Elementary Identification of Antagonistic Bacteria against Botrytis cinerea and Improving Biocontrol Effect Through Genetic Modification with Chitinase Gene[J]. China Biotechnology, 2010, 30(02): 71-76.

[15]	HUI Wei-Wei, DIAO Jian, WANG Zhen-Hu, JIA Tian. Expression of Chitinase Gene of HaSNPV, and Purification,Refolding of Its Recombinant Protein[J]. China Biotechnology, 2010, 30(02): 77-82.

Viewed

Full text

Abstract

Cited

Shared

Discussed