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| The Construct of Myostatin Gene Targeted Recipient Myoblast Cell |
| ZHANG Lei1|YANG Xing-yuan2|AN Xiao-rong2|CHEN Yong-fu2 |
| 1. Liaoning Medical University,Jinzhou121000,China
2. State Key Laboratory for Agrobiotechnology, College of Biology, China Agricultural University,Beijing100094,China |
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Abstract Abstract Myostatin (MSTN), a member of the transforming growth factor-β（TGF-β）superfamily, has been shown to be a negative regulator of myogenesis. Natural mutation in beef cattle causes double-muscling phenotypes. An investigation designed to knock out the myostatin gene by gene targeting in ovine myoblast cells were reported. Two promoter-trap targeting vectors MSTN-GFP and MSTN-Neo were constructed and used to transfect fetal and neonatal ovine primary myoblast cells. Both GFP expressing cells and drug resistant cells were obtained. Targeted cells expressing GFP were confirmed by PCR assay and drug resistant cells were characterized by PCR, Southern blot and DNA sequnce detection after growing into cell clones. That would supply nuleus transplantation donator for myostatin gene targetted somatic cloning sheep.
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Received: 15 December 2008
Published: 28 July 2009
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