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中国生物工程杂志

China Biotechnology
China Biotechnology
China Biotechnology  2019, Vol. 39 Issue (9): 41-49    DOI: 10.13523/j.cb.20190906
Orginal Article     
Normal Reference Range of Lymphocyte Subsets in Healthy Adults in Shanxi Province
HUI Yi-hua1,WANG Hai-na2,QI Yu-feng1,CAO Xue-ling3,GUAN Xue-mei3,DUAN Jing-jing2,DUAN Yi- jun2,WANG Yan- feng2,SU Wen2,**()
1 Second Hospital of Shanxi Medical University, Taiyuan 030001, China
2 Cancer Hospital Affiliated to Shanxi Medical University, Taiyuan 030013, China
3 Health Examination Center, Cancer Hospital Affiliated to Shanxi Medical University, Taiyuan 030013, China
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Abstract  

Objective: To establish normal reference range of peripheral blood lymphocyte subsets in healthy adults in Shanxi Province and provide theoretical basis for studying immunity status and assessing immune function in cancer patients.Methods: Selected 1 238 healthy adults in Shanxi Province and used six-color monoclonal antibody to detect and analysis absolute and percentage counts of peripheral blood lymphocyte subsets.Results: The expression of peripheral blood lymphocytes levels was determined, and it was found that CD3 +T、CD4 +T、CD8 +T、NK、CD19 cell percentage and absolute counts and CD4/CD8 ratio dissimilarity with a P value <0.05 was considered statistically significant ;and there was no statistical significance in CD8 + T cell percentage counts、CD4 +T cell and CD19 cell absolute counts between different genders,CD3 +T cell、CD8 +T cell、NK cell percentage and absolute counts、CD4 +T cell、CD19 cell percentage counts dissimilarity with a P value <0.05 was considered statistically significant. Conclusion: The refe -rence range of peripheral blood lymphocyte subsets in healthy adults in Shanxi Province was preliminarily established ,which provided reference for the evaluation of immunity function, tumor immunotherapy and diagnosis.



Key words： T lymphocyte subsets      Normal reference values      Flow cytometry      ShanXi Province     
Received: 21 August 2019      Published: 20 September 2019
ZTFLH:  Q26  
Corresponding Authors: Wen SU     E-mail: suwen30@yahoo.com
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Yi-hua HUI
Hai-na WANG
Yu-feng QI
Xue-ling CAO
Xue-mei GUAN
Jing-jing DUAN
Yi- jun DUAN
Yan- feng WANG
Wen SU
Cite this article:

HUI Yi-hua,WANG Hai-na,QI Yu-feng,CAO Xue-ling,GUAN Xue-mei,DUAN Jing-jing,DUAN Yi- jun,WANG Yan- feng,SU Wen. Normal Reference Range of Lymphocyte Subsets in Healthy Adults in Shanxi Province. China Biotechnology, 2019, 39(9): 41-49.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20190906     OR     https://manu60.magtech.com.cn/biotech/Y2019/V39/I9/41

T淋巴细胞亚型 相对计数(%) 绝对计数(个/μl) CD4/CD8 ratio
CD3 CD8 CD4 NK CD19 CD3 CD8 CD4 NK CD19
x? ±s 68.70±9.55 28.62±8.48 36.89±7.99 19.71±9.33 10.87±4.34 1 492.27±530.71 626.80±303.86 796.22±301.96 431.41±279.11 236.39±132.99 1.44±0.63
95%参考值范围 62.77~75.59 22.42~33.45 21.23~52.55 12.91~25.02 7.89~13.26 1 129.16~1 798.46 425.82~764.89 590.34~969.80 253.83~534.65 155.20~290.96 1.01~1.76
Table 1 Reference range of peripheral T lymphocyte subsets in 1 238 healthy adults
年龄组和性别
(n=例数)		 相对计数(%) 绝对计数(个/μl) CD4/CD8 比值
CD3 CD8 CD4 NK CD19 CD3 CD8 CD4 NK CD19
≤39(n=400)
男(n=93) 54.23~83.47 15.47~46.67 20.55~44.53 4.84~34.95 7.87~12.93 701.53~2 536.28 180.59~1 290.54 615.10~878.51 306.55~573.42 170.15~297.50 0.79~1.38
女(n=307) 66.51~77.36 23.14~34.43 25.72~50.79 10.78~20.90 8.38~13.07 1 224.68~1 841.61 456.25~764.84 633.79~1 000.84 202.96~460.34 167.01~301.76 1.03~1.77
40-59(n=560)
男(n=194) 63.01~73.36 21.8~,33.45 19.41~53.32 18.73~32.23 7.98~13.67 1 239.35~1 934.23 465.85~834.33 634.00~1 090.10 298.86~622.68 171.23~333.30 0.98~1.81
女(n=366) 63.97~76.45 22.28~31.82 33.62~43.94 12.52~23.38 8.33~13.35 1 101.68~1 627.46 393.35~675.24 587.77~947.39 222.69~460.16 153.47~265.50 1.15~1.83
≥60(n=278)
男(n=96) 42.14~87.76 10.18~52.25 14.06~48.93 3.68~43.29 5.00~11.89 994.71~1 820.33 431.13~908.25 499.31~912.97 358.85~732.63 99.33~238.96 0.71~1.55
女(n=182) 41.77~87.76 20.35~32.75 19.06~52.31 16.11~29.34 7.69~13.48 906.27~1 014.75 368.91~737.38 501.12~909.24 274.25~637.77 129.79~288.65 0.97~1.80
Table 2 95% reference range for healthy adults between different ages and genders
性别(n=例数) 相对计数(%) 绝对计数(个/μl) CD4/CD8 比值
CD3 CD8 CD4 NK CD19 CD3 CD8 CD4 NK CD19
女性(n=855) 69.44±9.45 28.16±8.07 38.10±7.48 18.75±8.79 11.10±4.31 1 445.45±500.20 588.22±265.67 790.28±288.54 395.88±259.02 232.79±135.63 1.49±0.61
男性(n=383) 67.03±9.58 29.65±9.27 34.21±8.45 21.83±10.15 10.37±4.37 1 596.78±580.41 712.94±364.15 809.49±329.95 510.74±305.18 244.43±126.70 1.32±0.65
P <0.001 0.14 <0.001 <0.001 0.004 <0.001 <0.001 0.69 <0.001 0.132 0.001
Table 3 Comparison of T lymphocyte subsets in peripheral blood of healthy adults of different genders
年龄组(n=例数) 相对计数(%) 绝对计数(个/μl) CD4/CD8 比值
CD3 CD8 CD4 NK CD19 CD3 CD8 CD4 NK CD19
≤39(n=400) 71.14±7.77 29.61±7.82 36.93±6.77 17.35±7.8 10.91±3.47 1 581.18±492.46 662.48±280.78 818.35±276.05 391.23±236.16 244.49±114.87 1.39±0.52
40~59(n=560) 69.33±8.97 27.72±8.02 38.18±8.05 18.96±8.60 11.10±4.04 1 490.18±527.25 597.74±281.73 818.31±320.45 410.11±258.78 237.06±115.52 1.53±0.67
≥60(n=278) 63.90±11.22 29.00±10.02 34.24±8.84 24.60±10.91 10.37±5.80 1 368.53±566.27 634.00±367.42 720.19±287.57 532.15±344.91 223.39±181.04 1.36±0.69
P <0.001 <0.001 0.004 <0.001 <0.001 <0.001 <0.001 0.69 <0.001 0.003 <0.001
Table 4 Comparison of T lymphocyte subsets in peripheral blood of healthy adults of different age groups
年龄组 P CD4/CD8 比值
相对计数(%) 绝对计数(个/μl)
CD3 CD8 CD4 NK CD19 CD3 CD8 CD4 NK CD19
27-39 40-59 0.014 0.002 0.086 0.037 1.000 0.001 <0.001 0.994 0.936 0.479 0.001
60-90 <0.001 0.749 <0.001 <0.001 0.011 <0.001 0.008 <0.001 0.011 <0.001 1.000
40-59 60-90 <0.001 0.255 <0.001 <0.001 0.004 0.001 1.000 <0.001 0.004 0.008 <0.001
Table 5 Pairwise comparison of P-value results among different age groups
Fig.1 The variation trend of peripheral blood lymphocyte subsets in different age groups Mean±standard deviation
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