Advance in Large-Scale Culture of Hybridoma Cells <i>in Vitro</i>

doi:10.13523/j.cb.20181010

China Biotechnology

2018, Vol. 38

Issue (10): 82-89 DOI: 10.13523/j.cb.20181010

Orginal Article

Advance in Large-Scale Culture of Hybridoma Cells in Vitro

Jing-jing SUN,Wei-wei ZHOU,Lei-ming ZHOU,Qiao-hui ZHAO(

),Gui-lin LI

Zhengzhou Autobio Diagnostic Co,LTD,Zhengzhou 450016,China

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Abstract

Monoclonal antibodies have shown great value in the field of biology and medical research. They are new reagents in immunoassays and are the guided weapons for biological therapy. As a diagnostic reagent in vitro, monoclonal antibody can give full play to its advantages, such as good specificity, high sensitivity, and more convenient for quality control, which is conducive to standardization .Traditionally,mouse ascites were used to prepare monoclonal antibodies. Now monoclonal antibodies are also being developed in large-scale cultures of hybridoma cells in vitro. In particular, the needs of monoclonal antibodies in the diagnosis and treatment of diseases have further promoted the development of invitro culture and production techniques for hybridoma cells. Due to the semi-adherent nature of the hybridoma cells, both in suspension and adherent culture, large-scale invitro culture of hybridoma cells can be performed. This article reviews the invitro culture techniques of hybridoma cells, including hollow fiber cell culture system and bioreactor cell culture sysytem, as well as the optimization of different culture methods.

Key words： Hybridoma Monoclonal antibody Bioreactor Hollow fiber

Received: 28 April 2018 Published: 09 November 2018

ZTFLH:

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Corresponding Authors: Qiao-hui ZHAO E-mail: zhaoqiaohui@autobio.com.cn

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	Jing-jing SUN
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	Gui-lin LI

Cite this article:

Jing-jing SUN,Wei-wei ZHOU,Lei-ming ZHOU,Qiao-hui ZHAO,Gui-lin LI. Advance in Large-Scale Culture of Hybridoma Cells in Vitro. China Biotechnology, 2018, 38(10): 82-89.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20181010 OR https://manu60.magtech.com.cn/biotech/Y2018/V38/I10/82

Fig.1 The costs in the stirred-tank bioreactor upstream section.

Fig.2 The cost distribution of operating costs in the hollow fiber bioreactor upstream section.

Table 1 MAb cultivation data (per batch) of the stirred-tank bioreactor (str) and hollow fiber bioreactor (HFB)

Fig.3 The cross section of in-culturing hollow fiber system^[8]

Table2 Previous studies hat guided process variable selection


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