|
|
|
| Effects of Self-assembling Peptide Hydrogel Scaffolds for Three-dimensional Culture on Biological Behavior and Capability of Myocardium Differentiation in Bone Marrow Mesenchymal Stem Cells |
| ZHAO Zheng-de1, CHEN Zhen-yin2, ZHANG Hui-nan2, GONG Jian-ping3, XU Shao-dan4, LUO Zhong-li2 |
1. The First Clinical College of Chongqing Medical University, Chongqing 400042, China;
2. The Molecular Medicine and Cancer Research Center, College of Basic Medical Sciences, Chongqing Medical University, Chongqingm 400042, China;
3. Central Hospital of Yiwu City, The Affiliated Yiwu Hospital of Wenzhou Medical University, Yiwu 322002, China;
4. Seventh People's Hospital of Zhengzhou City, Zhengzhou 450016, China |
|
|
|
Abstract Objective:To investigate the effect of BMSCs (bone marrow mesenchymal stem cells) at 3D (3-dimensional) culture microenvironment by peptide hydrogel scaffolds using self-assembling peptide GFS-4 on the biological behavior and the process of myocardium differentiation. Methods:The effect of peptides GFS-4 on self-assembling characteristics and the cell membrane disruptive were examined through Congo red staining and erythrocyte membrane lysis. Then, CCK8 and AO/EB staining were used to assess the difference in cell viability and apoptosis level between 2D (2-dimensional) and 3D microenvironment group.And the expression of MLC-2v and GATA-4 gene in the process of myocardium differentiation by Quantitative Real-Time PCR be analyzed, followed by BMSCs cultured at 2D and 3D environment for 3,5,7days. Results:The self-assembling peptide GFS-4 form a dense gel after 24 hours. There was no harmful for the cell membrane of the peptide before and after self-assembling.BMSCs at 3D culture environment showed that spherical shape, lower cell viability and lower apoptosis.When compared with the 2D culture environment group, the expression of MLC-2v and GATA-4 gene were respectively higher in the 3D environment group at 5 days and 7 days in the process of myocardium differentiation (P<0.05). Conclusions:The 3D culture environment constructed by peptide hydrogel scaffolds delayed BMSCs' proliferation and apoptosis rate, and also promoted the expression of MLC-2v and GATA-4 gene during the process of myocardium differentiation.
|
|
Received: 01 June 2017
Published: 15 November 2017
|
|
|
|
|
| [1] |
Wollert K C, Drexler H. Cell therapy for the treatment of coronary heart disease:a critical appraisal. Nat Rev Cardiol, 2010, 7(4):204-215.
|
|
|
| [2] |
Balogh L, Czuriga I, Kristof E, et al. Current practice and future perspectives of myocardial stem cell therapy. Orv Hetil, 2005, 146(20 Suppl 2):1110-1120.
|
|
|
| [3] |
Charwat S, Gyongyosi M, Lang I, et al. Role of adult bone marrow stem cells in the repair of ischemic myocardium:current state of the art. Exp Hematol, 2008, 36(6):672-680.
|
|
|
| [4] |
Luo Z, Zhang S. Designer nanomaterials using chiral self-assembling peptide systems and their emerging benefit for society. Chem Soc Rev, 2012, 41(13):4736-4754.
|
|
|
| [5] |
Nisbet D R, Williams R J. Self-assembled peptides:characterisation and in vivo response. Biointerphases, 2012, 7(1-4):2.
|
|
|
| [6] |
Luo Z, Yue Y, Zhang Y, et al. Designer D-form self-assembling peptide nanofiber scaffolds for 3-dimensional cell cultures. Biomaterials, 2013, 34(21):4902-4913.
|
|
|
| [7] |
岳媛媛,李萌萌,徐晓帆,等. 自组装短肽GFS-2构建新型三维细胞培养支架材料. 化学通报, 2015,78(3):273-276. Yue Y Y,Li M M,Xu X F, et al. Designer self-assembling peptide GFS-2 nanofiber scaffolds for 3D cell cultures. Chemistry, 2015,78(3):273-276.
|
|
|
| [8] |
罗忠礼, 陈振银, 岳媛媛,等. 自组装短肽GFS-4作为细胞三维培养及心肌梗死修复支架材料的研究. 生物医学工程学杂志, 2017,34(3):388-393. Luo Z L,Chen Z Y,Yue Y Y,et al Self-assembling peptide GFS-4 nanofiber scaffolds for three-dimensional cell cultures and myocardial infarction repair.Journal of Biomedical Engineering, 2017,34(3):388-393.
|
|
|
| [9] |
Utech S, Prodanovic R, Mao A S, et al. Microfluidic generation of monodisperse, structurally homogeneous alginate microgels for cell encapsulation and 3D cell culture. Adv Healthc Mater, 2015, 4(11):1628-1633.
|
|
|
| [10] |
Davidenko N, Schuster C F, Bax D V, et al. Evaluation of cell binding to collagen and gelatin:a study of the effect of 2D and 3D architecture and surface chemistry. J Mater Sci Mater Med, 2016, 27(10):148.
|
|
|
| [11] |
Arya N, Sardana V, Saxena M, et al. Recapitulating tumour microenvironment in chitosan-gelatin three-dimensional scaffolds:an improved in vitro tumour model. J R Soc Interface, 2012, 9(77):3288-3302.
|
|
|
| [12] |
Zhao J J, Liu X C, Kong F, et al. Bone marrow mesenchymal stem cells improve myocardial function in a swine model of acute myocardial infarction. Mol Med Rep, 2014, 10(3):1448-1454.
|
|
|
| [13] |
Wen Z, Zheng S, Zhou C, et al. Repair mechanisms of bone marrow mesenchymal stem cells in myocardial infarction. J Cell Mol Med, 2011, 15(5):1032-1043.
|
|
|
| [14] |
李琴,赵文婧,寇亚丽,等. 心肌组织裂解液诱导骨髓间充质干细胞向心肌样细胞的分化. 中国组织工程研究与临床康复, 2011, 15(10):1726-1730. Li Q, Zhao W J, Kou Y L, et al. Effects of myocardium lysate on the differentiation of rat bone marrow-derived mesenchymal stem cells into cardiac myocyte in vitro. Journal of Clinical Rehabilitative Tissue Engineering Research, 2011, 15(10):1726-1730.
|
|
|
| [15] |
Sheikh F, Lyon R C, Chen J. Functions of myosin light chain-2(MYL2) in cardiac muscle and disease. Gene, 2015, 569(1):14-20.
|
|
|
| [16] |
Xu H, Yi Q, Yang C, et al. Histone modifications interact with DNA methylation at the GATA4 promoter during differentiation of mesenchymal stem cells into cardiomyocyte-like cells. Cell Prolif, 2016, 49(3):315-329.
|
|
|
| [17] |
Moulin E, Cid J J, Giuseppone N. Advances in supramolecular electronics-from randomly self-assembled nanostructures to addressable self-organized interconnects. Adv Mater, 2013, 25(3):477-487.
|
|
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
