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| Molecular Mechanism of Inducing GLC-82 Cells Apoptosis by Ethanol Extract from Wedelia prostrate(Hook.et Arn.) Hemsl |
| DAI Li-ting1, WU Zhong-nan2, HUANG Xiang1, YANG Jie1, ZENG Hui-lan1, WANG Guo-cai2, JIANG Jian-wei1 |
1. Department of Biochemistry, Medical College, Jinan University, Guangzhou 510632, China;
2. Institute of Traditional Chinese Medicine and Nature Products, Jinan University, Guangzhou 510630, China |
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Abstract Objective:To study the antitumor mechanism of W40, a monomer purified from Wedelia prostrate (Hook.et Arn.) Hemsl. Methods:The effects of W40 on the cell proliferative of GLC-82 cells were detected by MTT assay and colony formation assay. The migratory abilities of GLC-82 cells were observed by wound healing assay. Cell apoptosis was evaluated by Annexin V-FITC/PI staining analysis.The levels of apoptosis-relative proteins and cell proliferation-related proteins, such as Caspase-3, PARP, Stat3 and ERK, were detected by Western blotting. Results:MTT assay showed that W40 had a significant cytotoxic effect on non-small cell lung cancer GLC-82 cells. Colony formation assays showed that W40 significantly inhibited GLC-82 cells proliferation.The migration of GLC-82 cells was inhibited by W40 in a dose-dependent manner. Flow cytometry showed that the apoptotic rate increased gradually in a concentration-dependent manner. W40 down-regulated Stat3 as decreasing p-Stat3 and downstream proteins of Bcl-2 and Mcl-1. At the same time, W40 up-regulated the expression of pro-apoptotic protein Bax, and increased the cleavaged Caspase-9,Caspase-3 and PARP. W40 also down-regulated BRAF/MAPK/ERK signal pathway as decreasing p-BRAF, p-MEK and p-ERK. Conclusions:W40 induced apoptosis by inhibiting BRAF/MAPK/ERK and Stat3 signaling pathways.
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Received: 23 May 2017
Published: 25 August 2017
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