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| The Expression Characteristics of IgM,C3 and LSZ in the Offspring from Female Ctenopharyngodon idella Immuned by Astragalus Polysaccharides |
| ZHOU Zhi-yu1, WANG Rong-hua1, JIN Sheng-zhen1, WANG Hong-quan1, XIAO Tiao-yi1,2 |
1. Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization, Hunan Agricultural University, Changsha 410128, China;
2. Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Changde 415000, China |
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Abstract In order to explore the feature of Astragalus polysaccharides on the female parent, studied the common humoral immune factors about IgM, C3 and LSZ in fish, and discussed the expression of humoral immune factors (IgM, C3, LSZ) and the transfer effects of immunological factors in the early developmental stage of female offspring. The results showed that the activities of IgM, C3 and LSZ in the blood of the experimental group were significantly higher than those of the control group. In the early stage of the progeny, the activity of the three immune factors proteins showed a trend from decline to rise. The activity of IgM protein in the experimental group was significantly higher than that in the control group, and raised 2.2 times, 1.7 times, 1.8 times in the period of the egg stage, 5d and 28d. The activity of C3 in the experimental group was significantly different from that in the control group, raised 1.9 times and 1.6 times during the period of the egg stage, 24h. The offspring has no significant difference between the experiment group and the control group. The activity of LSZ was consistent with the activity of IgM protein, increased by 2.4 times, 2.0 times, 1.9 times during the egg stage, 5d, 28d.The mRNA expression of three immune factors in the period of the egg stage and zygote period was significantly higher than that in the control group, But the expression of IgM and C3 can't find mRNAexpression during the period 24h~5d. However, the gene of three immune factors was up-regulated after 14d. After injecting with GCRV, the expression of IgM mRNA in the spleen and head kidney from the two-year-old grass carp was significantly higher than that of the control group. The results showed that the Astragalus polysaccharide could enhance the transformation of immune factors between generations, and play an important effect during the early development of filial generation.
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Received: 13 March 2017
Published: 25 July 2017
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