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| Regulation and Application in Metabolic Engineering of Bacterial Small RNAs |
| ZHAO Xiu-li, ZHOU Dan-dan, YAN Xiao-guang, WU Hao, CAIYIN Qing-gele, LI Yan-ni, QIAO Jian-jun |
| Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University;Key Laboratory of Systems Bioengineering, Ministry of Education;Syn Bio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin 300072, China |
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Abstract Metabolic engineering in bacteria requires optimization of gene expression levels to balance metabolic flux distributions and minimize accumulation of toxic intermediates towards an efficient biosynthesis of target products. Small non-coding RNAs (sRNAs) in bacteria can interact with their target mRNA by base-pairing to inhibit or activate the translation of their target genes. Since small RNAs play critical regulatory roles in many physiological processes, they are considered to be powerful tools for the tuning of target genes expression in bacterial metabolic engineering. Many artificial sRNAs are applied in metabolic engineering in recent years. The identification of sRNA targes genes, interaction between sRNAs and their targets and application of sRNAs in metabolic engineering were summarized.
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Received: 06 March 2017
Published: 25 June 2017
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