ANKRD49 Inhibits UV-induced Apoptosis of GC-1 Cells by Up-regulating Bcl-xL

doi:10.13523/j.cb.20170406

China Biotechnology

2017, Vol. 37

Issue (4): 40-47 DOI: 10.13523/j.cb.20170406

ANKRD49 Inhibits UV-induced Apoptosis of GC-1 Cells by Up-regulating Bcl-xL

BAI Xin-yan¹, WEN Li-min¹, WANG Yu-jing¹, WANG Hai-long², XIE Jun¹, GUO Rui¹

1 Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan 030001, China;
2 Department of Parasitology, Shanxi Medical University, Taiyuan 030001, China

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Abstract Objective:To study the effects of ANKRD49 on ultraviolet light (UV)-induced apoptosis of GC-1 cells. Methods:Apoptosis of GC-1 cells was induced by 40 J/m² UV for 2 minutes. The effect of ANKRD49 on mitochondrial membrane potential and nuclear condensation of GC-1 cells was detected by flow cytometry and Hoechst33258 staining, respectively. The levels of PARP, Cleaved-Caspase-3, Bcl-xL and Bax were measured by Western blotting. Results:The results of flow cytometry showed that the rates of decline of mitochondrial membrane potential and apoptosis in ANKRD49 transfected group were significantly lower than those in other groups(P<0.05). Hoechst33258 staining results demonstrated that the percentage of apoptosis in ANKRD49 transfected group was significantly lower than that in other groups (P<0.05). Western blotting results showed that the expression of Cleaved-PARP and Cleaved-Caspase-3 protein in ANKRD49 over-expressed group was significantly lower than that in other tested groups (P<0.05), while the expression of Bcl-xL protein was significantly higher in ANKRD49 over-expressed group than that in control groups. Conclusion:Overexpression of ANKRD49 could inhibit the apoptosis of GC-1 cells via up-regulating Bcl-xL expression.

Key words： ANKRD49 Cell apoptosis Bcl-xL GC-1cells

Received: 25 October 2016 Published: 25 April 2017

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BAI Xin-yan, WEN Li-min, WANG Yu-jing, WANG Hai-long, XIE Jun, GUO Rui. ANKRD49 Inhibits UV-induced Apoptosis of GC-1 Cells by Up-regulating Bcl-xL. China Biotechnology, 2017, 37(4): 40-47.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20170406 OR https://manu60.magtech.com.cn/biotech/Y2017/V37/I4/40

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