The Study of Osteogenic Induction of Type Ⅰ Collagen /Poly(caprolactone)/Attapulgite Composite Scaffold Materials <em>in Vitro</em>

doi:10.13523/j.cb.20160504

China Biotechnology

2016, Vol. 36

Issue (5): 27-33 DOI: 10.13523/j.cb.20160504

The Study of Osteogenic Induction of Type Ⅰ Collagen /Poly(caprolactone)/Attapulgite Composite Scaffold Materials in Vitro

ZHANG Xiao-min^1,2, WANG Shi-yong², LI Gen², ZHAO Hong-bin^1,2

1. School of Life Science and Technology, Gansu Agriculture University, Lanzhou 730070, China;
2. Institute of Orthopedics, General Hospital of Lanzhou Military Command of the PLA, Lanzhou 730050, China

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Abstract

Objective:To study the biocompatibility and osteoinductivity of typeⅠcollagen/poly (caprolactone)/attapulgite composite scaffolds in vitro. Methods:The ColⅠ/PCL/ATP composite scaffolds materials of three different content (0%, 10%, and 30%wt) ATP were fabricated by the solution perfusion-salt-leaching process. D1 cells were co-cultured with the materials for 7d or 1~6d, the biocompatibility of scaffold was evaluated by scanning electron microscopy, Phalloidin and H&E staining and CCK-8, respectively. D1 cells were co-cultured with the materials for 7d, 14d, and 21d; the relative expression of bone-related genes including Runx-2, Osterix, ALP, Col I, OPN, and OC were measured by RT-qPCR, and evaluated osteogenic effect comparison of three scaffolds. Results:Three scaffolds had suitable cell adhesion and appreciation properties, especially 30%wt ATP materials. The results of RT-qPCR show that Runx-2 expression of 30%wt ATP was significant increased in 7d, but decreased in 14d, 21d; and ALP expression was significant increased in 14d, but significant decreased in 21d; and there were significant increased in the relative mRNA levels of Osterix, Col I, OPN, OC, compared with the 0% and 10%wt ATP materials (P<0.05), which had time and dose dependent manner. Conclusion:ColⅠ/PCL/ATP composite scaffolds may be used as an ideal scaffold for bone tissue engineering.

Key words： Attapulgite Osteoinductivity Biocompatibility Composite scaffold biomaterials

Received: 11 January 2016 Published: 25 May 2016

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R318.08

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ZHANG Xiao-min, WANG Shi-yong, LI Gen, ZHAO Hong-bin. The Study of Osteogenic Induction of Type Ⅰ Collagen /Poly(caprolactone)/Attapulgite Composite Scaffold Materials in Vitro. China Biotechnology, 2016, 36(5): 27-33.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20160504 OR https://manu60.magtech.com.cn/biotech/Y2016/V36/I5/27

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