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China Biotechnology
China Biotechnology  2016, Vol. 36 Issue (4): 18-23    DOI: 10.13523/j.cb.20160403
The Application and Safety Assessment of Stacked Transgenic Plant
WANG Xu-jing1, ZHANG Xin1, LIU Pei-lei2, WANG Zhi-xing1
1. Biotechnology Research Institute, CAAS, Beijing 100081, China;
2. Development Center of Science and Technology, Ministry of Agriculture of the People's Republic of China, Beijing 100122, China
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Stacked transgenic plant which harboring two or more foreign genes, can meet the diverse needs of the growers and bring multiple benefits, has become an important trend in the current development of genomic modified crops. Different countries of the world take the different safety assessment model for stacked transgenic plant according to its own regulation idea and the view on genetic stability and gene interaction. The application of the stacked transgenic plant, safety assessment model of different countries were reviewed, and the suggestions on safety assessment of stacked transgenic plant in China were made also.

Key wordsTransgenic plant      Application      Breeding stack      Safety assessment     
Received: 13 January 2016      Published: 15 February 2016
ZTFLH:  Q789  
Cite this article:

WANG Xu-jing, ZHANG Xin, LIU Pei-lei, WANG Zhi-xing. The Application and Safety Assessment of Stacked Transgenic Plant. China Biotechnology, 2016, 36(4): 18-23.

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[1] 刘培磊,李宁,程金根. 不同国家和地区复合性状转基因植物安全评价管理的比较. 农业科技管理, 2008, 27(3): 23-27. Liu P L, Li N, Cheng J G. Comparison of safety assessment of stacked transgenic plant in different country and region. Management of Agricultural Science and Technology, 2008, 27(3): 23-27.
[2] Halpin C. Gene stacking in transgenic plants——the challenge for 21st century plant biotechnology. Plant Biotech J, 2005, 3 : 141-155.
[3] James Clive. 2014年全球生物技术/转基因植物商业化发展态势. 中国生物工程杂志, 2015, 35(1): 1-14. James C. Global status of commercialized biotech/GM crops in 2014.China Biotech, 2015, 35(1): 1-14.
[4] James Clive. 2011年全球生物技术/转基因植物商业化发展态势. 中国生物工程杂志, 2012, 32(1): 1-14. James C. Global status of commercialized biotech/GM crops in 2011. China Biotech, 2012, 32(1): 1-14.
[5] World Health Organization. Application of the Principles of Substantial Equivalence to the Safety Evaluation of Foods or Food Components from Plants Derived by Modern Biotechnology. Geneva: World Health Organization 1995/FNU/FOS/95.1.
[6] CropLife International. Regulation of plant biotechnology products containing two or more traits combined by conventional plant breeding. 2005, [7] Food and Agricultural Organization of the United Nations/World Health Organization. Joint FAO/WHO expert consultation on biotechnology and food safety. .
[8] International Seed Federation. Genetically modified crops and plant breeding. .
[9] EPA. Biopesticides registration action document ——Bacillus thuringiensis plant-incorporated Protectants. http: //www.epa. gov/oppbppd1/biopesticides/pips/bt-brad. htm.
[10] Canadian Food Inspection Agency. Directive 94-08 (Dir 94-08): Assessment Criteria for Determining Environmental Safety of Plants With Novel Traits.
[11] Canadian Food Inspection Agency. Regulating the Environmental Release of Stacked Plant Products in Canada.
[12] Office of the Gene Technology Regulator of Australia. Policy on Licensing of Plant GMOs in which Different Genetic Modifications have been Combined (or 'Stacked') by Conventional Breeding.
[13] Food Safety Commission. Regarding safty assessment of crossing of genetically modified plants. 2004-1-29.
[14] EFSA. Guidance document of the scientific panel on genetically modified organisms for the risk assessment of genetically modified plants and derived food and feed. The EFSA Journal, 2004,99:1-94.
[15] The European Association for Bioindustries. Safety assessment of GM crops: Document 5- Evaluation of crops containing GM events combined by traditional breeding .
[16] Anon. Risk Assessment of Plants Containing Genetic Modification Events Combined by Crossing- for public consultation. gmo/gmo_consultations/1035_en.html.
[17] McCouch S. Diversifying selection in plant breeding. PLoS Biolology, 2004, 2: 1507-1512.
[18] Duvick D N. Plant breeding, an evolutionary concept. Crop Sci, 1996, 36: 539-548.
[19] Hajjar R, Hodgkin T. The use of wild relatives in crop improvement: a survey of developments over the last 20 years. Euphytica, 2007, 156: 1-13.
[20] Fernie A R, Tadmor Y, Zamir D. Natural genetic variation for improving crop quality. Curr Opin Plant Biol, 2006, 9:196-202.
[21] Groszmann M, Greaves I K, Albertyn Z I, et al. Changes in 24-nt siRNA levels in Arabidopsis hybrids suggest an epigenetic contribution to hybrid vigor. Proc Natl Acad Sci USA, 2011, 108: 2617-2622.
[22] Xu X, Pan S, Cheng S, et al. Genome sequence and analysis of the tuber crop potato. Nature, 2011, 475: 189-195.
[23] Parrott W, Chassy B, Ligon J, et al. Application of food and feed safety assessment principles to evaluate transgenic approaches to gene modulation in crops. Food Chem Toxicol, 2010, 48: 1773-1790.
[24] Cellini F, Chesson A, Colquhoun I, et al. Unintended effects and their detection in genetically modified crops. Food Chem Toxicol, 2004, 42: 1089-1125.
[25] National Research Council. Safety of Genetically Engineered Foods: Approaches to Assessing Unintended Health Effects. Washington, DC, USA: National Academies Press, 2004.
[26] Pilacinski W, Crawford A, Downey R, et al. Plants with genetically modified events combined by conventional breeding: an assessment of the need for additional regulatory data. Food Chem Toxicol, 2011, 49(1):1-7.
[27] Weber N, Halpin C, Hannah L C, et al. Editor's choice: Crop genome plasticity and its relevance to food and feed safety of genetically engineered breeding stacks. Plant Physiol, 2012, 160(4):1842-1853.
[28] Steiner H Y, Halpin C, Jez J M, et al. Editor's choice: Evaluating the potential for adverse interactions within genetically engineered breeding stacks. Plant Physiol, 2013, 161(4):1587-1594.

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