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中国生物工程杂志

China Biotechnology
China Biotechnology  2015, Vol. 35 Issue (10): 20-26    DOI: 10.13523/j.cb.20151003
    
SATB2 Promotes Bone Morphogenetic Protein 9-induced Osteogenic Differentiation of Mesenchymal Stem Cells
FENG Qiao-ling, WANG Yu-feng, LIU Xiao-hua, JI Cai-xia, LUO Jin-yong
Key laboratory of Laboratory Medical Diagnostics, Ministry of Education, Chongqing Medical University, Chongqing 400016, China
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Abstract  

Objective: To analyze the effect of SATB2 on BMP9-induced osteogenic differentiation of C2C12 mesenchymal stem cells. Methods: C2C12 cells were treated with ad-BMP9, and then the expressions of SATB2 at the gene and protein level were detected with RT-PCR and Western blot, respectively. Afterwards C2C12 cells were treated with ad-SATB2 or/and BMP9, then ALP activity was detected by quantitative and staining assay, and calcium deposition was detected by Alizarin Red S staining. Results: BMP9 can enhance the expression of SATB2 at the gene level and at the protein level in C2C12 cells. Both ALP activity and calcium deposition of C2C12 cells treated with adenovirus SATB2 or/and BMP9 expressed higher than those of controls. Conclusions: SATB2 can promote BMP9-induced osteogenic differentiation of C2C12 mesenchymal stem cells



Key wordsBone morphogenetic protein 9      C2C12 cells      Special AT-rich sequence binding protein-2     
Received: 24 May 2015      Published: 25 October 2015
ZTFLH:  Q254  
Cite this article:

FENG Qiao-ling, WANG Yu-feng, LIU Xiao-hua, JI Cai-xia, LUO Jin-yong. SATB2 Promotes Bone Morphogenetic Protein 9-induced Osteogenic Differentiation of Mesenchymal Stem Cells. China Biotechnology, 2015, 35(10): 20-26.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20151003     OR     https://manu60.magtech.com.cn/biotech/Y2015/V35/I10/20

[1] Pittenger M F, Mackay A M, Beck S C, et al. Multilineage potential of adult human mesenchymal stem cells. Science, 1999, 284(5411):143-147.
[2] Horwitz E M, Prockop D J, Fitzpatrick L A, et al. Transplantability and therapeutic effects of bone marrow-derived mesenchymal cells in children with osteogenesis imperfecta. Nat Med, 1999, 5(3):309-313.
[3] Jiang Y, Jahagirdar B N, Reinhardt R L, et al. Pluripotency of mesenchymal stem cells derived from adult marrow. Nature, 2002, 418(6893):41-49.
[4] Tseng Y H, Kokkotou E, Schulz T J, et al. New role of bone morphogenetic protein 7 in brown adipogenesis and energy expenditure. Nature, 2008, 454(7207):1000-1004.
[5] Cheng H, Jiang W, Phillips F M, et al. Osteogenic activity of the fourteen types of human bone morphogenetic proteins (BMPs). J Bone Joint Surg Am, 2003, 85-A(8):1544-1552.
[6] Govender S, Csimma C, Genant H K, et al. Recombinant human bone morphogenetic protein-2 for treatment of open tibial fractures: a prospective, controlled, randomized study of four hundred and fifty patients. J Bone Joint Surg Am, 2002, 84-A(12):2123-2134.
[7] Luo J, Tang M, Huang J, et al. TGFbeta/BMP type I receptors ALK1 and ALK2 are essential for BMP9-induced osteogenic signaling in mesenchymal stem cells. J Biol Chem, 2010, 285(38):29588-29598.
[8] Sinha K M, Zhou X. Genetic and molecular control of osterix in skeletal formation. J Cell Biochem, 2013, 114(5):975-984.
[9] Lee K S, Kim H J, Li Q L, et al. Runx2 is a common target of transforming growth factor beta1 and bone morphogenetic protein 2, and cooperation between Runx2 and Smad5 induces osteoblast-specific gene expression in the pluripotent mesenchymal precursor cell line C2C12. Mol Cell Biol, 2000, 20(23):8783-8792.
[10] Dobreva G, Chahrour M, Dautzenberg M, et al. SATB2 is a multifunctional determinant of craniofacial patterning and osteoblast differentiation. Cell, 2006, 125(5):971-986.
[11] Zhang P, Men J, Fu Y, et . Contribution of SATB2 to the stronger osteogenic potential of bone marrow stromal cells from craniofacial bones. Cell Tissue Res, 2012, 350(3):425-437.
[12] Savarese F, Davila A, Nechanitzky R, et al. Satb1 and Satb2 regulate embryonic stem cell differentiation and Nanog expression. Genes Dev, 2009, 23(22):2625-2638.
[13] Asanoma K, Kubota K, Chakraborty D, et al. SATB homeobox proteins regulate trophoblast stem cell renewal and differentiation. J Biol Chem, 2012, 287(3):2257-2268.
[14] Tu Q, Valverde P, Li S, et al. Osterix overexpression in mesenchymal stem cells stimulates healing of critical-sized defects in murine calvarial bone. Tissue Eng, 2007, 13(10):2431-2440.
[15] He T C, Zhou S, Da C L, et al. A simplified system for generating recombinant adenoviruses. Proc Natl Acad Sci U S A, 1998, 95(5): 2509-2514.
[16] Mendez-Ferrer S, Michurina T V, Ferraro F, et al. Mesenchymal and haematopoietic stem cells form a unique bone marrow niche. Nature, 2010, 466(7308):829-834.
[17] Chambers I, Colby D, Robertson M, et al. Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. Cell, 2003, 113(5):643-655.
[18] Chang H Y. Anatomic demarcation of cells: Genes to patterns. Science, 2009, 326(5957):1206-1207.
[19] Dong W, Zhang P, Fu Y, et al. Roles of SATB2 in site-specific stemness, autophagy and senescence of bone marrow mesenchymal stem cells. J Cell Physiol, 2015, 230(3):680-690.
[20] Gong Y, Qian Y, Yang F, et al. Lentiviral-mediated expression of SATB2 promotes osteogenic differentiation of bone marrow stromal cells in vitro and in vivo. Eur J Oral Sci, 2014, 122(3):190-197.
[21] Asanoma K, Kubota K, Chakraborty D, et al. SATB homeobox proteins regulate trophoblast stem cell renewal and differentiation. J Biol Chem, 2012, 287(3):2257-2268.
[22] Chang H Y, Chi J T, Dudoit S, et al. Diversity, topographic differentiation, and positional memory in human fibroblasts. Proc Natl Acad Sci USA, 2002, 99(5):12877-12882.

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