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中国生物工程杂志

China Biotechnology
China Biotechnology
China Biotechnology  2015, Vol. 35 Issue (9): 122-127    DOI: 10.13523/j.cb.20150917
    
Metabolic Control Fermentation Mechanism and Breeding Strategies of Cytidine Excessive Biosynthesis in Bacillus amyloliquefaciens
WU Qing, LIU Hui-yan, FANG Hai-tian, HE Jian-guo, HE Xiao-guang, YU Li-nan, WANG Meng-jiao
School of Agriculture Ningxia University, Yinchuan 750021, China
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Abstract  

Cytidine is good intermediates of anti-tumor and antiviral, also can be used as health food ingredients.The metabolic regulatory mechanism of cytidine biosynthesis in Bacillus amyloliquefaciens was discussed and the breeding strategy of constructing cytidine high-yielding strain was summarized. The focuses were on elaborating the six breeding strategies, such as improving the pyrimidine operon transcriptional level of Bacillus amyloliquefaciens, enhancing cytidine anabolic pathways and central metabolic pathways to cytidine synthesis, blocking degradation pathway of cytidine, increasing the pentose phosphate pathway to the sub-flow of cytidine synthesis pathway, reducing the bypass pathway and accelerating cytidine secretion.Thereby, the strategies of breeding high yield strain were proposed, which could provide some ideas for breeding cytidine high-yielding strains, and solve the existing problems in the processing of cytidine industrial production.

Key words： Bacillus amyloliquefaciens      Cytidine      Metabolic regulation mechanism      Breeding strategy     
Received: 25 March 2015      Published: 25 September 2015
ZTFLH:  Q819  
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Cite this article:

WU Qing, LIU Hui-yan, FANG Hai-tian, HE Jian-guo, HE Xiao-guang, YU Li-nan, WANG Meng-jiao. Metabolic Control Fermentation Mechanism and Breeding Strategies of Cytidine Excessive Biosynthesis in Bacillus amyloliquefaciens. China Biotechnology, 2015, 35(9): 122-127.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20150917     OR     https://manu60.magtech.com.cn/biotech/Y2015/V35/I9/122


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