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中国生物工程杂志

China Biotechnology
China Biotechnology  2015, Vol. 35 Issue (8): 16-22    DOI: 10.13523/j.cb.20150803
    
Effect of Ginsenoside Rg1 on Bone Marrow Hematopietic Function of Aging Model Rats and Its Biological Mechanism
SHAO Yue, ZHANG Li-heng, RAN Rui-tu, SUN Jia-zheng, ZHANG Jing, ZHANG Meng-si, JIA Dao-yong, ZHANG Yan-yan, WANG Ya-ping
Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, Chongqing 400016, China
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Abstract  

Objective: To explore the effects and the relative mechanism of ginsenoside Rg1 on bone marrow hematopietic function in aging model rats, and provide theoretical and experimental evidences for searching effective natural medicine of antiaging or protecting hematopietic function. Methods: Male SD rats were randomly divided into normal group, Rg1 normal group, aging model group and Rg1 aging model group. Aging model group: the rats were administrated with D-galactose 120mg/kg,qd×42 by subcutaneous way; Rg1 aging group: the rats were also given D-galactose of the same dose and time as aging model group, and from the 15th day on, rats were treated with Rg1 20 mg/kg qd×28 by intraperitoneal way; Normal control group: The rats were injected with same volume saline qd×42 by subcutaneous way; Rg1 normal group: the rats were given saline with the same volume,qd×14, then received Rg1 20 mg/kg qd×28 by same way. After 2 days of finishing the treatment, peripheral blood was collected to detect the number of leukocytes,analyzes classification of leukocyte and advanced glycosylation end products (AGEs); The femur was taken to count the number of bone marrow mononuclear cells(BMMCs) in each femur, the proliferation of BMMCs was detected; Senescence-accociated β-galactosidase (SA-β-Gal) stain was analyzed aging BMMCs; multipotential hematopoietic progenitor (CFU-Mix) was cultured to observe the colony formation of BMMCs; the expression of senescence-associated protein P21 and P53 in BMMCs was assayed by Western blotting; peritoneal macrophage was extracted and detected the phagocytosis of the macrophage by colorimetric method. Results: Compared with the aging model group, Rg1 aging model group can significantly increase the number of leukocytes, regulate the percentage of granulocyte and lymphocytes, the proportion of CD8+T cells and CD4+T cells; the number of BMMCs in each femur; decrease the ratio of SA-β-Gal positive BMMCs,the amounts of AGEs;enhance the ability of BMMCs to form CFU–Mix; down-regulate the expression of senescence- associated protein P21 and P53;promote the phagocytic index of peritoneal macrophage. Conclusion: The hematopoietic cells are obviously induced senescence by treating with D-galactose, ginsenoside Rg1 has a significantly antiaging or protective effect on hematopoietic cells. It is suggested that the mechanism may be Rg1 protecting hematopietic cells by regulating p53/p21 signaling pathway.



Key wordsGinsenoside Rg1      Aging model      Bone marrow      Hematopoietic cell      Mechanism     
Received: 28 March 2015      Published: 25 August 2015
ZTFLH:  R329.2  
Cite this article:

SHAO Yue, ZHANG Li-heng, RAN Rui-tu, SUN Jia-zheng, ZHANG Jing, ZHANG Meng-si, JIA Dao-yong, ZHANG Yan-yan, WANG Ya-ping . Effect of Ginsenoside Rg1 on Bone Marrow Hematopietic Function of Aging Model Rats and Its Biological Mechanism. China Biotechnology, 2015, 35(8): 16-22.

URL:

http://manu60.magtech.com.cn/biotech/10.13523/j.cb.20150803     OR     http://manu60.magtech.com.cn/biotech/Y2015/V35/I8/16


[1] Wagner W M, Ouyang Q, Sekeri-Pataryas K, et al. Basic biology and clinical impact of immunosenescence. Biogerontology, 2004, 5(1): 63-66.

[2] Chu S, Zhang J.New achievements in ginseng research and its future prospects.Chinese Journal of Integrative Medicine, 2009, 15(6):403-408.

[3] 周玥, 杨斌, 姚欣, 等. 人参皂苷 Rg1 延缓造血干细胞衰老与 p16INK4a 表达关系的研究.中国中药杂志,2011,36(5):608-613. Zhou Y,Yang B,Yao X,et al. Experimental study of relationship between effect of ginsenoside Rg1 to delay hematopoietic stem cell senescence and expression of p16 INK4a.China Journal of Chinese Materia Medica, 2011, 36(5):608-613.

[4] Shang Y Z,Gong M Y,Zhou X X, et al.Improving effects of SSF on memory deficits and pathological changes of neural and immunological systems in senescent mice.Acta Pharmacol Sin, 2001,22(12):1078-1083.

[5] 秦红兵,杨朝晔,范忆江,等.半乳糖诱导衰老小鼠模型的建立与评价.中国组织工程研究与临床康复,2009,13(7):1275-1278. Qin H B, Yang Z Y, Fan Y J,et al. Establishment and evaluation of aging models induced by D-galactose in mice, Journal of Clinical Rehabilitative Tissue Engineering Research, 2009, 13(7):1275-1278.

[6] 彭彬, 陈茂山, 蒲莹, 等.人参皂苷Rg1延缓D-半乳糖大鼠脑衰老作用及机制的初步研究.重庆医科大学学报, 2011, 0(4):419-422. Peng B,Chen M S,Pu Y,et al. Anti- aging effects of ginsenoside Rgl and it's mechanisms on brain aging rats induced by D-galactose. Journal of Chongqing Medical University, 2011, 36(4):419-422.

[7] 郑敏, 王亚平.当归多糖对人髓系多向造血祖细胞增殖分化的影响及其机理研究.解剖学杂志,2002,25(2):105-109. Zheng M,Wang Y P. Study on biological mechanism of angelica polysaccharide regulation on human early multipotentiai progenitor cell. Chinese Journal of Anatomy, 2002,25(2):105-109.

[8] Kobari L,Giarratana M C,Poloni A,et al. Flt3 ligand,MGDF,Epo and GCSF enhance ex vivo expansion of hematopoietic cell compartments in the presence of SCF,IL -3 and IL-6.Bone Marrow Transpl, 1998, 2l(8):759-767.

[9] 孙缅恩, 杜冠华.晚期糖基化终产物的病理意义及其机制.中国药理学通报,2002,18(3):246-249. Sun M E, Du G H. Pathological significance and mechanism of advanced glycation end-products. Chinese Pharmacological Bulletin, 2002, 18(3):246-249.

[10] 杨继乐, 张莉, 王莉.单核-巨噬细胞的分化和功能研究进展.细胞与分子免疫学杂志, 2014,30(11):1213-1216. Yang J L,Zhang L,Wang L. The research progress of mononuclear macrophages' differentiation and function. Journal of Cellular and Molecular Immunology, 2014, 30(11):1213-1216.

[11] Ohtani N, Yamakoshi K, Takahashi A, et al. The p16INK4a-RB pathway: molecular link between cellular senescence and tumor suppression.The Journal of Medical Investigation, 2004, 51(3-4):146-153.

[12] Chen X, Zhang W, Gao Y, et al.Senescence-like changes induced by expression of p21(waf1cip1)in NIH3T3 cell line.Cell Res, 2002, 12(3-4):229-233.

[13] Molchadsky A, Shats I, Goldfinger N, et al. p53 plays a role in mesenchymal differentiation programs, in a cell fate dependent manner.PLoS One, 2008, 3(11):3707-3722.

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