Effect of Ginsenoside Rg1 on Bone Marrow Hematopietic Function of Aging Model Rats and Its Biological Mechanism

doi:10.13523/j.cb.20150803

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China Biotechnology ›› 2015, Vol. 35 ›› Issue (8) : 16-22. DOI: 10.13523/j.cb.20150803

Effect of Ginsenoside Rg1 on Bone Marrow Hematopietic Function of Aging Model Rats and Its Biological Mechanism

SHAO Yue, ZHANG Li-heng, RAN Rui-tu, SUN Jia-zheng, ZHANG Jing, ZHANG Meng-si, JIA Dao-yong, ZHANG Yan-yan, WANG Ya-ping

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Abstract

Objective: To explore the effects and the relative mechanism of ginsenoside Rg1 on bone marrow hematopietic function in aging model rats, and provide theoretical and experimental evidences for searching effective natural medicine of antiaging or protecting hematopietic function. Methods: Male SD rats were randomly divided into normal group, Rg1 normal group, aging model group and Rg1 aging model group. Aging model group: the rats were administrated with D-galactose 120mg/kg,qd×42 by subcutaneous way; Rg1 aging group: the rats were also given D-galactose of the same dose and time as aging model group, and from the 15th day on, rats were treated with Rg1 20 mg/kg qd×28 by intraperitoneal way; Normal control group: The rats were injected with same volume saline qd×42 by subcutaneous way; Rg1 normal group: the rats were given saline with the same volume,qd×14, then received Rg1 20 mg/kg qd×28 by same way. After 2 days of finishing the treatment, peripheral blood was collected to detect the number of leukocytes,analyzes classification of leukocyte and advanced glycosylation end products (AGEs); The femur was taken to count the number of bone marrow mononuclear cells(BMMCs) in each femur, the proliferation of BMMCs was detected; Senescence-accociated β-galactosidase (SA-β-Gal) stain was analyzed aging BMMCs; multipotential hematopoietic progenitor (CFU-Mix) was cultured to observe the colony formation of BMMCs; the expression of senescence-associated protein P21 and P53 in BMMCs was assayed by Western blotting; peritoneal macrophage was extracted and detected the phagocytosis of the macrophage by colorimetric method. Results: Compared with the aging model group, Rg1 aging model group can significantly increase the number of leukocytes, regulate the percentage of granulocyte and lymphocytes, the proportion of CD8⁺T cells and CD4⁺T cells; the number of BMMCs in each femur; decrease the ratio of SA-β-Gal positive BMMCs,the amounts of AGEs;enhance the ability of BMMCs to form CFU–Mix; down-regulate the expression of senescence- associated protein P21 and P53;promote the phagocytic index of peritoneal macrophage. Conclusion: The hematopoietic cells are obviously induced senescence by treating with D-galactose, ginsenoside Rg1 has a significantly antiaging or protective effect on hematopoietic cells. It is suggested that the mechanism may be Rg1 protecting hematopietic cells by regulating p53/p21 signaling pathway.

Key words

Ginsenoside Rg1 / Aging model / Bone marrow / Hematopoietic cell / Mechanism

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SHAO Yue, ZHANG Li-heng, RAN Rui-tu, SUN Jia-zheng, ZHANG Jing, ZHANG Meng-si, JIA Dao-yong, ZHANG Yan-yan, WANG Ya-ping . Effect of Ginsenoside Rg1 on Bone Marrow Hematopietic Function of Aging Model Rats and Its Biological Mechanism[J]. China Biotechnology, 2015, 35(8): 16-22 https://doi.org/10.13523/j.cb.20150803

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