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Fermentative Production of L-lactic Acid from Wastepaper by Recombinant Escherichia coli WL204 |
XIONG Yuan-yuan, LU Chuan-dong, TAO Ye, ZHAO Jin-fang |
Hubei Cooperative Innovation Center for Industrial Fermentation, Key Laboratory of Fermentation Engineering Ministry of Education, Hubei University of Technology, Wuhan 430068, China |
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Abstract Previous studies, a recombinant E.coli WL204 was constructed,which contained a chromosomal integrated ldhL gene and possesses the capability of homofermentation of L-lactic acid using xylose as substrate. Wastepaper was selected as feedstock to evaluate the fermentative production of lactic acid by recombinant E.coli WL204.The wastepaper chips were treated with 1:6-1:14(w/v)H2SO4, and then incubated 60 h at 50℃ with cellulase. The resultant hydrolyzates were detoxified with dried Ca(OH)2, which could remove most of furfural and HMF. Fermentation of the detoxified hydrolyzate of wastepaper with E.coli WL204 produced 31g L-lactic acid from 100g dry wastepaper and the lactic acid yield coefficient was 79%.These results demonstrated that WL204 has the potential for homofermentative production of L-lactic acid using cellulosic biomass derived substrates.
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Received: 04 March 2015
Published: 25 May 2015
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[1] Gullón B, Yáñez R, Alonso J L, et al. L-Lactic acid production from apple pomace by sequential hydrolysis and fermentation. Bioresource Technology, 2008, 99 (2): 308-319.
[2] John R P, Anisha G S, Nampoothiri K M, et al. Direct lactic acid fermentation: focus on simultaneous saccharification and lactic acid production. Biotechnol Adv, 2009, 27 (2): 145-152.
[3] Narita J, Nakahara S, Fukuda H, et al. Efficient production of L-(+)-lactic acid from raw starch by Streptococcus bovis 148. Journal of Bioscience and Bioengineering, 2004, 97 (6): 423-425.
[4] Tanaka T, Hoshina M, Tanabe S, et al. Production of D-lactic acid from defatted rice bran by simultaneous saccharification and fermentation. Bioresour Technol, 2006, 97 (2): 211-217.
[5] Kojima Y, Yoon S L. Improved enzymatic hydrolysis of waste paper by ozone pretreatment. Journal of Material Cycles and Waste Management, 2008, 10(2): 134-139.
[6] Zhang Y, Chen X, Luo J, et al. An efficient process for lactic acid production from wheat straw by a newly isolated Bacillus coagulans strain IPE22. Bioresource Technology, 2014, 158(2): 396-399.
[7] Zhang Y, Chen X, Qi B, et al. Improving lactic acid productivity from wheat straw hydrolysates by membrane integrated repeated batch fermentation under non-sterilized conditions. Bioresource Technology, 2014, 163(4): 160-166.
[8] 张晶晶,万金泉. 废纸与葡萄糖的亚临界水解动力学. 中华纸业,2011,32(6):46-50. Zhang J J,Wan J Q. Subcritical hydrolytic dynamics of waste paper and glucose. China Pul P& Paper Industry,2011,32(6):46-50.
[9] Budhavaram N K, Fan Z. Production of lactic acid from paper sludge using acid-tolerant, thermophilic Bacillus coagulan strains. Bioresource Technology, 2009, 100(23): 5966-5972.
[10] Park E Y, Anh PN, Okuda N. Bioconversion of waste office paper to L (+)-lactic acid by the filamentous fungus Rhizopus oryzae. Bioresource Technology, 2004, 93(1): 77-83.
[11] Ouyang J, Ma R, Zheng Z, et al. Open fermentative production of l-lactic acid by Bacillus sp. strain NL01 using lignocellulosic hydrolyzates as low-cost raw material. Bioresource Technology, 2013,135:475-480.
[12] Marques S, Santos J A, Gírio F M, et al. Lactic acid production from recycled paper sludge by simultaneous saccharification and fermentation. Biochemical Engineering Journal,2008, 41(3): 210-216.
[13] Saito K, Hasa Y, Abe H. Production of lactic acid from xylose and wheat straw by Rhizopus oryzae. Journal of Bioscience and Bioengineering, 2012, 114(2): 166-169.
[14] Zhao J, Xu L, Wang Y, et al. Homofermentative production of optically pure L-lactic acid from xylose by genetically engineered Escherichia coli B. Microbial Cell Factories, 2013, 12 (1): 1-57.
[15] Wang Y, Manow R, Finan C, et al. Adaptive evolution of nontransgenic Escherichia coli KC01 for improved ethanol tolerance and homoethanol fermentation from xylose. Journal of Industrial Microbiology & Biotechnology, 2011, 38(9): 1371-1377.
[16] Sluiter A, Hames B, Ruiz R, et al. Laboratory Analytical Procedure: Determination of Structural Carbohydrates and Lignin in Biomass. Golden, Colorado: National Renewable Energy Laboratory, 2011: 1-15.
[17] 侯玉林. 不同预处理对再生植物纤维结构及水解反应影响的研究. 广州:华南理工大学, 环境科学与工程学院, 2011. Hou Y L. Effect of Pretreatments on Structure and Hydrolysis of Recycled Fibers. Guangzhou:South China University of Technology, College of Environmental Science and Engineering, 2011.
[18] 黄峰. 大肠杆菌利用水稻脆性秸秆发酵产乙醇的研究. 武汉:湖北工业大学,生物工程学院,2014. Huang F.Study on Fermentation of Cellulosic Ethanol by Escherichia coli Using Brittle Rice Straw.Wuhan:Hubei University of Technology,College of Biological Engineering, 2014.
[19] Gutiérrez T, Ingram L O, Preston J F. Purification and characterization of a furfural reductase (FFR) from Escherichia coli strain LYO1—an enzyme important in the detoxification of furfural during ethanol production. Journal of Biotechnology, 2006, 121 (2): 154-164.
[20] Mazumdar S, Bang J, Oh M K. L-Lactate production from seaweed hydrolysate of Laminaria japonica using metabolically engineered Escherichia coli. Applied Biochemistry and Biotechnology, 2014, 172(4): 1938-1952.
[21] Wang Y, Li K, Huang F, et al. Engineering and adaptive evolution of Escherichia coli W for L-lactic acid fermentation from molasses and corn stee Pliquor without additional nutrients. Bioresource Technology, 2013, 148(8): 394-400.
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