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| Fermentative Production of L-lactic Acid from Wastepaper by Recombinant Escherichia coli WL204 |
| XIONG Yuan-yuan, LU Chuan-dong, TAO Ye, ZHAO Jin-fang |
| Hubei Cooperative Innovation Center for Industrial Fermentation, Key Laboratory of Fermentation Engineering Ministry of Education, Hubei University of Technology, Wuhan 430068, China |
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Abstract Previous studies, a recombinant E.coli WL204 was constructed,which contained a chromosomal integrated ldhL gene and possesses the capability of homofermentation of L-lactic acid using xylose as substrate. Wastepaper was selected as feedstock to evaluate the fermentative production of lactic acid by recombinant E.coli WL204.The wastepaper chips were treated with 1:6-1:14(w/v)H2SO4, and then incubated 60 h at 50℃ with cellulase. The resultant hydrolyzates were detoxified with dried Ca(OH)2, which could remove most of furfural and HMF. Fermentation of the detoxified hydrolyzate of wastepaper with E.coli WL204 produced 31g L-lactic acid from 100g dry wastepaper and the lactic acid yield coefficient was 79%.These results demonstrated that WL204 has the potential for homofermentative production of L-lactic acid using cellulosic biomass derived substrates.
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Received: 04 March 2015
Published: 25 May 2015
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