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| Isolation, Identification and Fermentation Optimization of Antagonistic Bacillus subtilis KC-5 |
| LIU Hui-li1, LI Yuan-yuan2, JU Rui-cheng2, ZHAO Hong-tao2, YANG Qing1 |
1. College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China;
2. Qingdao Bright Moon Seaweed Group Co., Ltd., Qingdao 266400, China |
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Abstract In order to screen biocontrol bacteria being antagonistic to some plant pathogens, Bacillus subtilis KC-5 was isolated from the vegetable soil to inhibit a variety of pathogenic fungi with Tablet confrontation France, and the observations about inhibition of mycelia suggested that the strains KC-5 can inhibit the activity of Fusarium oxysporum, Fusarium moniliforme, Fusarium proliferatum and Fusarium solani. By analyzing the morphological, physiological and biochemical characteristics and 16S rRNA sequence, the strain was identified as Bacillus subtilis and its fermentation medium was optimized.
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Received: 29 November 2013
Published: 25 March 2014
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