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中国生物工程杂志

China Biotechnology
China Biotechnology
China Biotechnology  2020, Vol. 40 Issue (1-2): 146-153    DOI: 10.13523/j.cb.1905007
Orginal Article     
Application of Peptide Nucleic Acid in Virus Detection and Therapy
SUN Heng1,WANG Jing1,ZENG Ling-gao2,WANG Jian-hua1,**()
1 Bioengineering College of Chongqing University, Chongqing 400044, China
2 Chongqing Institute for Food and Drug Control, Chongqing 401121, China
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Abstract  

Peptide nucleic acid (PNA) is an oligonucleotide analog that replaces the sugar phosphate backbone with polypeptide backbone, and known as a third-generation antisense nucleic acid. The electrically neutral polypeptide backbone structure of PNA enables it to retain the high target affinity of the sugar phosphate chain oligonucleotide, and at the same time, it has stronger enzyme stability and thermal stability than the sugar phosphate backbone, which has become the hotspot in the research of oligonucleotide analogues. On the one hand, the rapid, effective and accurate detection performance of PNA on virus replication and mutation level makes it of great significance for further treatment of diseases. On the other hand, the life cycle of the virus is regulated by the PNA based on its sequence specificity and dose dependency at the gene level. Therefore, as to effective inhibit the survival and replication of the virus in the host cell. The latest progress and mechanism of PNA in the detection of different viruses and the treatment of viral diseases has been reviewed based on the literature of the past decade, which is expected to provide new guidances and ideas for the development of clinical products of peptide nucleic acids.

Key words： Peptide nucleic acid      Detection      Antiviral     
Received: 07 May 2019      Published: 27 March 2020
ZTFLH:  Q819  
Corresponding Authors: Jian-hua WANG     E-mail: wjh@cqu.edu.cn
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Cite this article:

SUN Heng,WANG Jing,ZENG Ling-gao,WANG Jian-hua. Application of Peptide Nucleic Acid in Virus Detection and Therapy. China Biotechnology, 2020, 40(1-2): 146-153.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.1905007     OR     https://manu60.magtech.com.cn/biotech/Y2020/V40/I1-2/146

Fig.1 The structure of PNA and DNA
PNA名称 序列(N→C端) 靶标 检测限 应用 参考文献
PANArray ACATCATCCATATAAC 逆转录酶M204V 102cps/ml HBV [12]
PNA-YIDD1 GTTATATCGATGATGTGG 逆转录酶204 0.01% HBV [11]
PNA-QCM TCCTTTTTT-OOO-TTTTTTCCT 基因组DNA序列 8.6pg/L HBV [14]
PNA-3a CACACATATCACCC 3a核心/E1区 1.8×10-12mol HCV [15]
PNA-SAM ATGTACCCCATGAGGTCGGC 核心/E1区 9.5pg/μl HCV [16]
PNA-HCV CCCAATTATACTTGCGGC M2基因 5% H1N1 [17]
bisPNA-AZO TCTCTTCC-AZO-CCTTCTCTTCCAGGA NS基因 6.0×104~6.0×105pfu/ml H1N1 [18]
PNA-ISH CTGGCTTTAATTTTA NL43WC001基因pol区 — HIV [19]
Table 1 PNA sequence for virus detection
PNA名称 序列(5'-3') 靶标 IC50 应用 参考文献
PNA 2052 TAGACGTAAAGATAC 衣壳化信号ε 10nmol DHBV [22]
PNA 2053 GCAATGTAGACGTAA 衣壳化信号ε 10nmol DHBV [22,25]
PNA-DR GCAGAGGTGAA pgRNA DR序列 — HBV [27]
肽-asPNA-寡核苷酸支架 AGGTGAAAAAGTTGCAT HBV DNA 1 814~1 830 — HBV [28]
PNA 6 TACGAGACCTCCCGGGG IRES 314~330 — HCV [30]
PNA 10 GTGCTCATGG IRES的IV环区域 54nmol HCV [31]
PNA-SL3 AGATGGAGCCACC X-RNA SL结构1~13 — HCV [32]
PNA-SL3-15 TAAGATGGAGCCACC X-RNA SL结构1~15 — HCV [32,41]
dbPNA TLTTTQTLLL dsRNA — 多种IAV亚型 [34]
TiO2·PL·DNA/PNA GCAAAAGCAGGGTAGA NP基因 3μg/ml H3N2 [35]
PNA-TAR TCCCAGGCTCAGATCT TAR 0.8μmol HIV [37-39]
PNA J3U5 TCGCGGCTTCATACA 3'-UTR 10 931~10 945 — JEV [40]
PNA J3U6 TCTCGCGGCTTCATACA 3'-UTR 10 931~10 947 — JEV [40]
PNA J3U2 TCGGCGCTCTGTGCC 3'-UTR 10 928~10 942 — JEV [40-41]
FS PNA AGCCCTGTAGACGAC PRF 13 458~13 472 4.4μmol SARS-CoV [41]
Table 2 PNA sequence for virus therapy
Fig.2 The structure of PNA analogs
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