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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2021, Vol. 41 Issue (6): 45-49    DOI: 10.13523/j.cb.2102016
综述     
重组酶介导扩增技术及其在病原微生物快速检测中的应用进展*
马巧妮1,王萌1,**(),朱兴全2,**()
1 中国农业科学院兰州兽医研究所 家畜疫病病原生物学国家重点实验室 甘肃省动物寄生虫病重点实验室 兰州 730046
2 山西农业大学动物医学学院 太谷 030801
Research Advances in Recombinase-aided Amplification Technology and Its Application in Rapid Detection of Pathogenic Microorganisms
MA Qiao-ni1,WANG Meng1,**(),ZHU Xing-quan2,**()
1 State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agriculture Sciences,Lanzhou 730046, China
2 College of Veterinary, Shanxi Agricultural University, Taigu 030801, China
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摘要：

建立快速的病原学诊断方法对动物疫病的预防和控制、公共卫生安全等方面具有重要意义。重组酶介导扩增法(RAA)是一种新型恒温体外核酸扩增技术,在体外较低温度下就可以实现对DNA或RNA的快速扩增。RAA具有操作简便、快速、准确、节能、便捷等优点。重组酶、单链结合蛋白和DNA聚合酶是该技术的三大核心物质,利用这三种物质可替代传统PCR的热循环解链过程。该技术将会在病原微生物检测方面发挥重要作用。对RAA技术及其在病原检测方面的应用进行了综述,以期为相关领域的研究提供参考。
关键词： 重组酶介导扩增法(RAA);  病原微生物;  诊断;  检测    
Abstract:

The establishment of rapid diagnostic methods for pathogens is of great significance for the prevention and control of animal epidemics and public health safety in China. Recombinase-aided amplification (RAA) is a novel isothermal amplification technology in which rapid amplification of DNA or RNA can be carried out at low temperatures, and the entire RAA reaction is simple, rapid, accurate, power-saving and convenient. There are three core substances of this technology, including recombinase, single-stranded DNA binding protein and DNA polymerase, which can replace the traditional PCR thermal cycle chain decomposition process. Therefore, the RAA technology will play a more and more important role in the detection of pathogenic microorganisms. The research advances in the detection of pathogens by the RAA technology was reviewed, which may provide reference for further relevant studies.
Key words: Recombinase-aided amplification (RAA)    Pathogenic microorganisms    Diagnosis    Detection
收稿日期: 2021-02-15 出版日期: 2021-07-06
ZTFLH:  Q819  
基金资助: * 国家自然科学基金(32002306);山西省“1331工程”(20211331-13);中国农业科学院科技创新工程(CAAS-ASTIP-2016-LVRI-03)
通讯作者: 王萌,朱兴全     E-mail: wangmeng02@caas.cn;xingquanzhu1@hotmail.com
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引用本文:

马巧妮,王萌,朱兴全. 重组酶介导扩增技术及其在病原微生物快速检测中的应用进展*[J]. 中国生物工程杂志, 2021, 41(6): 45-49.

MA Qiao-ni,WANG Meng,ZHU Xing-quan. Research Advances in Recombinase-aided Amplification Technology and Its Application in Rapid Detection of Pathogenic Microorganisms. China Biotechnology, 2021, 41(6): 45-49.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.2102016    或    https://manu60.magtech.com.cn/biotech/CN/Y2021/V41/I6/45

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