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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2021, Vol. 41 Issue (2/3): 1-6    DOI: 10.13523/j.cb.2011023
研究报告     
PHP14通过与Vimentin相互作用影响TGF-β诱导的肝细胞AML-12上皮-间质转化*
徐安健1,**(),李艳萌1,乌姗娜2,张蓓1,姚静怡1
1 首都医科大学附属北京友谊医院科研实验中心 北京 100875
2 首都医科大学附属北京友谊医院临床检验中心 北京 100875
PHP14 Plays a Role in Epithelial-Mesenchymal Transition of AML-12Cell Through Interaction with Vimentin
XU An-jian1,**(),LI Yan-meng1,WU Shan-na2,ZHANG Bei1,YAO Jing-yi1
1 Experimental Center, Beijing Friendship Hospital, Beijing 100875, China
2 Clinical Laboratory Center, Beijing Friendship Hospital, Beijing 100875, China
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摘要：

目的: 探讨PHP14在上皮-间质转化中的作用及其可能作用机制。方法: 建立了研究上皮-间质转化的细胞模型小鼠肝细胞AML-12。在AML-12中过表达PHP14,通过光学显微镜进行形态学观察,应用实时荧光定量PCR和蛋白质印迹检测AML-12上皮-间质转化中相关标志物的表达情况。通过免疫共沉淀检测PHP14与Vimentin的相互作用情况。结果: 小鼠肝细胞AML-12可作为研究上皮-间质转化的较好细胞模型。在AML-12中过表达PHP14可促进AML-12细胞的上皮-间质转化,并增强其运动能力。过表达PHP14可上调Vimentin的蛋白质水平,而对其他上皮-间质转化相关标志物表达没有影响。进一步的研究发现,PHP14可与Vimentin相互作用。结论: PHP14通过与Vimentin相互作用,稳定Vimentin的蛋白质水平,从而参与并调节上皮-间质转化。
关键词： PHP14;  上皮-间质转化;  AML-12 Vimentin;  细胞运动    
Abstract:

Objective: To investigate the role of PHP14 in epithelial-mesenchymal transition and its possible mechanism. Methods: A cell model was established to study epithelial-mesenchymal transition in mouse hepatocytes AML-12. Overexpression of PHP14 in AML-12. Morphological observation was carried out by light microscope. The expression of related markers in epithelial-mesenchymal transition of AML-12 was detected by real-time fluorescent quantitative PCR and Western blot. The interaction between PHP14 and Vimentin was detected by immunoprecipitation. Results: Mouse hepatocyte AML-12 can be used as a good cell model to study epithelial-mesenchymal transition. Overexpression of PHP14 in AML-12 can promote epithelial-mesenchymal transition and enhance the motility of AML-12 cells. Overexpression of PHP14 upregulated Vimentin, but had no effect on the expression of other epithelial-mesenchymal transition related markers. Further studies showed that PHP14 could interact with Vimentin. Conclusion: PHP14 can stabilize Vimentin protein level and participate in and regulate epithelial-mesenchymal transition, by interacting with Vimentin.
Key words: PHP14    Epithelial-mesenchymal transition    AML-12 Vimentin    Cell migration
收稿日期: 2020-11-11 出版日期: 2021-04-08
ZTFLH:  Q819  
基金资助: * 北京市自然科学基金资助项目(7202034)
通讯作者: 徐安健     E-mail: xuanjian@ccmu.edu.com
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徐安健
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引用本文:

徐安健,李艳萌,乌姗娜,张蓓,姚静怡. PHP14通过与Vimentin相互作用影响TGF-β诱导的肝细胞AML-12上皮-间质转化*[J]. 中国生物工程杂志, 2021, 41(2/3): 1-6.

XU An-jian,LI Yan-meng,WU Shan-na,ZHANG Bei,YAO Jing-yi. PHP14 Plays a Role in Epithelial-Mesenchymal Transition of AML-12Cell Through Interaction with Vimentin. China Biotechnology, 2021, 41(2/3): 1-6.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.2011023    或    https://manu60.magtech.com.cn/biotech/CN/Y2021/V41/I2/3/1

图1  TGF-β可诱导小鼠肝细胞系AML-12发生显著的上皮-间质转化
图2  PHP14可促进AML-12在TGF-β作用下的上皮-间质转化及体外运动能力
图3  在AML-12细胞中过表达PHP14可提高TGF-β作用下Vimentin蛋白的表达
图4  PHP14可与vimentin相互作用
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