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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2020, Vol. 40 Issue (6): 93-99    DOI: 10.13523/j.cb.2002046
综述     
工业微生物发酵过程中pH调控研究进展 *
高小朋1,**(),何猛超1,许可2,3,**(),李春1,2,3
1 延安大学生命科学学院 延安 716000
2 清华大学化学工程系 北京 100084
3 北京理工大学化学与化工学院 合成生物系统研究所 北京 100081
Research Progress on pH Regulation in the Process of Industrial Microbial Fermentation
GAO Xiao-peng1,**(),HE Meng-chao1,XU Ke2,3,**(),LI Chun1,2,3
1 School of Life Science, Yan’an University, Yan’an 716000, China
2 Department of Chemical Engineering, Tsinghua University, Beijing 100084, China
3 Department of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China
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摘要：

pH是影响微生物生长的重要因素。工业发酵过程中,pH调控波动产生酸/碱胁迫,进而影响到微生物细胞的生长及目标产物的积累。阐述了工业微生物在发酵体系pH波动时所发生的生理变化,总结了目前工业发酵中pH调控的主要策略及各自的优缺点,并指出了未来pH调控的发展方向,以期为发酵工业pH调控提供新的思路。
关键词： 工业微生物;  酸碱胁迫;  pH调控策略    
Abstract:

PH is an important factor affecting the growth of microorganisms. In the process of industrial fermentation, the fluctuation of pH regulation could lead to acid / alkali stresses, which affects the growth of microbial cells and the accumulation of target products. In this review, the physiological changes of industrial microorganisms in the pH fluctuation of fermentation system were described, and the main strategies of pH regulation in industrial fermentation and the comparison of them were summarized. Furthermore, the development direction of pH regulation in the future was pointed out, as it can provide new ideas for pH regulation in fermentation industry.
Key words: Industrial microorganism    Acid/alkali stress    pH regulation
收稿日期: 2020-02-27 出版日期: 2020-06-23
ZTFLH:  Q819  
基金资助: * 国家自然科学基金(21868038)
通讯作者: 高小朋,许可     E-mail: gaoxiaopengyd@163.com;xuke528@tsinghua.edu.cn
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高小朋
何猛超
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引用本文:

高小朋,何猛超,许可,李春. 工业微生物发酵过程中pH调控研究进展 *[J]. 中国生物工程杂志, 2020, 40(6): 93-99.

GAO Xiao-peng,HE Meng-chao,XU Ke,LI Chun. Research Progress on pH Regulation in the Process of Industrial Microbial Fermentation. China Biotechnology, 2020, 40(6): 93-99.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.2002046    或    https://manu60.magtech.com.cn/biotech/CN/Y2020/V40/I6/93

微生物种类 最低pH 最适pH 最高pH
Escherichia coli 4.3 6.5~7.5 9.5[16]
Bacillus subtilis 4.5 6.5~7.5 8.5[17]
Staphylococcus aureus 4.2 7.0~7.5 9.3[18]
Aspergillus niger 1.5 5.0~6.0 9.0[19]
Lactobacillus acidophilus 4.0 5.8~6.6 6.8[20]
Corynebacterium glutamate 5.5 7.0~8.5 10.0[21]
Saccharomyces cerevisiae 4.0~4.5 7.5[22]
表1  部分常见微生物的最适pH
图1  微生物胞内pH的动态平衡机制
图2  微生物胞内质子泵的作用机制[35]
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