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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2017, Vol. 37 Issue (11): 83-88    DOI: 10.13523/j.cb.20171111
研究报告     
产酸丙酸杆菌耐酸菌株的选育及其应用
孙丹1, 徐国霞2, 王自强2, 王云山2, 张利平1, 苏志国2
1. 河北大学生命科学学院 保定 071002;
2. 中国科学院过程工程研究所生化工程国家重点实验室 北京 100190
Screening and Application of Acid-resistant Strains of Propionibacterium acidipropionici
SUN Dan1, XU Guo-xia2, WANG Zi-qiang2, WANG Yun-shan2, ZHANG Li-ping1, SU Zhi-guo2
1. College of Life Science, Hebei University, Baoding 071002, China;
2. The State Key Lab of Biochemical Engineering, The Institute of Process Engineering of the Chinese Academy of Sciences, Beijing 100190, China
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摘要： 为了解除微生物发酵生产丙酸过程中代谢产物（丙酸）对菌体生长的抑制作用，以实验室保藏的产酸丙酸杆菌（耐30g/L丙酸）为出发菌株P-0，通过丙酸压力筛选获得了一株耐10g/L丙酸的产酸性能良好的菌株P-10，降低了发酵过程中丙酸对菌体生长的抑制作用。菌株P-10做摇瓶发酵，发酵周期168h，丙酸浓度为49.66g/L，产酸速率为0.30g/（L·h），较出发菌株P-0提高了53.04%；7L发酵罐实验表明，菌株P-10发酵周期168h，丙酸浓度为55.63g/L，产酸速率0.33g/（L·h）。同时对菌株P-10做二次接种实验，结果表明，84h为二次接种最适时间段，且84h进行二次接种时，丙酸浓度提高了17.77%，二次接种实验不但有利于有机酸的积累，而且可以提高菌株的产酸能力和耐酸能力；经过选育的菌株P-10具有优良的产酸稳定性，有利于菌种的工业化生产和应用，同时对后续的发酵分离耦合具有重要意义。
关键词： 产酸丙酸杆菌;  丙酸;  压力筛选;  二次接种    
Abstract: In order to relieve the inhibitory effect of the metabolites (propionic acid) on the growth of the cells during the production of propionic acid by microbial fermentation, the laboratory storage of Propionibacterium acidipropionici (resistant to 30g/L propionic acid) P-0 as the original strain, a strain of P-10 with good acid-producing ability of 10g/L propionic acid was obtained by propionic acid pressure screening, which reduced the inhibitory effect of propionic acid on cell growth during fermentation.The strain P-10 was subjected to shake flask fermentation, the fermentation cycle was 168h, the concentration of propionic acid was 49.66g/L, the rate of acid production was 0.30g/(L·h), which was 53.04% higher than that of the strain P-0. The experiment of 7L fermentor showed that the fermentation period of the strain P-10 was 168h, the concentration of propionic acid was 55.63g/L, the acid production rate was 0.33g/(L·h). At the same time, the second inoculation experiment was carried out on the strain P-10. The results showed that the optimum period was 84h, and the concentration of propionic acid increased by 17.77% at the second time. The secondary inoculation experiment was not only beneficial to the organic acid but also can improve the acid production capacity and acid resistance of the strains. The tested strain P-10 has excellent acidic stability, which is beneficial to the industrial production and application of the strain and is of great significance to the subsequent fermentation separation and coupling.
Key words: Propionibacterium acidipropionici    Pressure screening    Propionic acid    Secondary inoculation
收稿日期: 2017-04-24 出版日期: 2017-11-15
ZTFLH:  Q815  
基金资助: 国家"973"计划（2013CB733604）、国家自然科学基金（21506227）资助项目
通讯作者: 王云山     E-mail: yswang@ipe.ac.cn
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引用本文:

孙丹, 徐国霞, 王自强, 王云山, 张利平, 苏志国. 产酸丙酸杆菌耐酸菌株的选育及其应用[J]. 中国生物工程杂志, 2017, 37(11): 83-88.

SUN Dan, XU Guo-xia, WANG Zi-qiang, WANG Yun-shan, ZHANG Li-ping, SU Zhi-guo. Screening and Application of Acid-resistant Strains of Propionibacterium acidipropionici. China Biotechnology, 2017, 37(11): 83-88.

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https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20171111    或    https://manu60.magtech.com.cn/biotech/CN/Y2017/V37/I11/83

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