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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2021, Vol. 41 Issue (6): 50-59    DOI: 10.13523/j.cb.2102020
综述     
分子生物学方法提高电活性微生物胞外电子传递效率的研究进展*
邵映芝1,车鉴2,程驰1,**(),江志阳3,薛闯1,**()
1 大连理工大学生物工程学院 大连市合成生物学应用转化工程技术研究中心 大连 116024
2 大连鑫玉龙海洋生物种业科技股份有限公司 大连 116222
3 中国科学院沈阳应用生态研究所 沈阳 110016
Advances in Molecular Biological Methods to Improve Extracellular Electron Transport Efficiency of Electroactive Microorganisms
SHAO Ying-zhi1,CHE Jian2,CHENG Chi1,**(),JIANG Zhi-yang3,XUE Chuang1,**()
1 Dalian University of Technology, School of Bioengineering, Engineering Research Center of Application and Transformation for Synthetic Biology, Dalian 116024, China
2 Dalian Xinyulong Marine Biological Seed Technology Co., Ltd., Dalian 116222, China
3 Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China
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摘要：

微生物细胞与电极之间的胞外电子传递效率是限制微生物电化学技术发展的关键因素,而分子生物学的发展为提高胞外电子传递效率带来了光明前景。从四种具有代表性的纯培养电活性微生物(奥奈达希瓦氏菌、铜绿假单胞菌、硫还原地杆菌和工程大肠杆菌)和混合培养电活性微生物出发,综述了利用分子生物学手段改造几种电活性微生物的研究成果,阐明了针对特异的电活性微生物,如何采取相应的分子生物学手段提高其胞外电子传递的效率,并展望了未来的研究方向。
关键词： 胞外电子传递;  电活性微生物;  分子生物学;  电活性生物膜    
Abstract:

The efficiency of extracellular electron transfer between microbial cells and electrodes is a key factor limiting the development of microbial electrochemical technology, and the development of molecular biology has brought bright prospects for improving the efficiency of extracellular electron transfer. The results of engineering four representative electroactive microorganisms (Shewanella oneidensis MR-1, Pseudomonas aeruginosa, Geobacter sulfurreducens and engineered Escherichia coli) in pure culture and mixed culture by means of molecular biology are reviewed. How molecular biology methods adopt corresponding improvement strategies for different electroactive microorganisms are explained, and future research directions are prospected.

Key words: Extracellular electron transfer    Electroactive microorganism    Molecular biology    Electroactive biofilm
收稿日期: 2021-02-22 出版日期: 2021-07-06
ZTFLH:  Q819  
基金资助: * 国家自然科学基金(21878035);国家自然科学基金(21808026);中国博士后面上基金(2019M661100)
通讯作者: 程驰,薛闯     E-mail: cheng.chi@dlut.edu.cn;xue.1@dlut.edu.cn
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引用本文:

邵映芝,车鉴,程驰,江志阳,薛闯. 分子生物学方法提高电活性微生物胞外电子传递效率的研究进展*[J]. 中国生物工程杂志, 2021, 41(6): 50-59.

SHAO Ying-zhi,CHE Jian,CHENG Chi,JIANG Zhi-yang,XUE Chuang. Advances in Molecular Biological Methods to Improve Extracellular Electron Transport Efficiency of Electroactive Microorganisms. China Biotechnology, 2021, 41(6): 50-59.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.2102020    或    https://manu60.magtech.com.cn/biotech/CN/Y2021/V41/I6/50

图1  三种电活性微生物的胞外电子传递机制
EAM Modification strategy Results of modification Reference
S. oneidensis
MR-1 		Overexpression of the NAD+ synthesis gene The maximum power output density was 4.4 times higher than that in the wild type and the coulomb efficiency was 1.5 times higher [24]
Overexpression of flaxin synthesis gene cluster (ribDCBAE) and metal reductase gene cluster (mtrCAB) The maximum current density in MFC increased by about 110% [25]
Increased electroactive biofilm thickness of MFC anode The maximum output power density is 18.8 times higher than that of the wild type [28]
Expressing a c-di-GMP synthase to increase cytoplasmic c-di-GMP level The high c-di-GMP strain had a higher Fe(III) reduction rate (21.58 vs 11.88 pmol/L of Fe(III)/h cell) and greater expression of genes that code for the proteins involved in the Mtr pathway [30]
Intelligent resource allocation based on quorum sensing The efficiency of extracellular electron transfer improved by 4.8 times [31]
P. aeruginosa Knocking out the global regulator RpoS Enhanced the formation of biofilm on the electrode [37]
Heterologous expression of global regulator gene IrrE Maximum power density increased by approximately 71% [39]
G. sulfurreducens Overexpression of polysaccharide synthetic gene GSU1501 The maximum current is 22.2% higher than the control strain [17]
Overexpression of PilA gene from G. metallireducens in G. sulfurreducens The conductivity is 5 000 times that of G. sulfurreducens and 1 million times that of G. uraniireducens [48]
Engineered E. coli Nine different soluble phenazineredox mediators that support efficient EET were added. A maximum mediated current density of(7.9 ± 0.9) μA/cm 2 with NR, followed by(6.2 ±0.6) μA/cm 2 with pyocyanin, and about 3.0 μA/cm 2 with no mediators [13]
Expression of the exogenous gene encoding NDH-2 inE. coli BL21(DE3) The total NAD(H) increased by 1.32 times [51]
Multiple-knockout of central metabolism genes The maximum output power is 1.5 times higher than the wild type [49]
Expression of FAD synthetic genes Compared with the parent strain, the yield of succinic acid produced by fumaric acid increased by about 60% [52]
Introduced the Mtr pathway into E. coli cells by expressing ccmABCDEFGH from E. coli Succinic acid yield increased by 6.32 mmol/L and coulomb efficiency increased by about 113% [50]
Inhibition of colanic acid synthesis gene (wcaF) Effectively control the overgrowth of biofilm [55]
Mixed culture E. coli-B. subtilis-S. oneidensis Glucose (11 mmol/L, total 0.28 g) was converted to electricity for more than 15 days with high energy conversion efficiency (up to 55.7%) [56]
K. pneumoniae-S. oneidensis The maximum power density is up to 19.9 mW/m2 [57]
E. coli-E. coli-S. oneidensis The electricity production exceeds 350 mV, and the electricity production time exceeds 100 hours [58]
表1  提高EAM的EET效率汇总表
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