运用BioID技术筛选S100A7互作蛋白*

王俊豪,孔菲,李允广,刘进,胡恩泽,王睿,黄凌云,肖雪媛

Screening of S100A7 Interacting Proteins Using BioID Technology

Jun-hao WANG,Fei KONG,Yun-guang LI,Jin LIU,En-ze HU,Rui WANG,Ling-yun HUANG,Xue-yuan XIAO

图5 S100A7与 AnxA2免疫荧光共定位验证

Fig.5 Co-localization verification of S100A7 and AnxA2 S100A7 overexpression plasmid (pcDNA3.1 - S100A7) and AnxA2 overexpression plasmid (pCMV14 AnxA2 flag). S100A7 specific antibody showed S100A7 with red fluorescence; FLAG tag antibody showed AnxA2 with green fluorescence; DAPI specific staining of DNA showed nuclei with blue fluorescence. S100A7 and AnxA2 were co-located in the cytoplasm and cell membrane