斑马鱼hoxa1a基因调控颅面骨骼发育的功能研究*

武秀知,王宏杰,祖尧

Functional Study of hoxa1a Regulating Craniofacial Bone Development in Zebrafish

Xiu-zhi WU,Hong-jie WANG,Yao ZU

图2 成功得到hoxa1a的纯合突变体斑马鱼,并发现面部骨骼发育畸形

Fig.2 A homozygous hoxa1a-/- zebrafish with abnormal facial development was successfully established (a) Comparison of sequencing peak maps of wildtype, hoxa1a+/- and hoxa1a-/- zebrafish. The red arrows indicate the position at which the mutation starts, and the red boxes indicate the inserted bases (b) Phenotype observations showed that the wildtype and heterozygous mutant had normal faces, while the homozygous mutant faces skeletal were abnormal at 5 dpf. As shown by the red arrow, Scale bar: 100 μm